caa a22 4500 38640058X CHVBK 20180307112157.0 cr unu---uuuuu 161130e198906 xx s 000 0 eng 10.1017/S0031182000061564 doi S0031182000061564 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000061564 Major sperm protein and actin genes in free-living and parasitic nematodes [Elektronische Daten] The DNA from a number of free-living and parasitic nematode species was examined to determine the genomic number and distribution of DNA sequences encoding two evolutionary conserved proteins; the major sperm protein (MSP) and nematode actin. Ascaris and Caenorhabditis MSP cDNA sequences and Ascaris genomic actin sequences were used to probe Southern blots of Eco RI and Hin d III digested nematode DNA. The number of MSP genes varied widely between the 1 MSP gene in Ascaris and the 60 MSP genes in Caenorhabditis. Filarial nematodes appeared to have 1—4 MSP genes while the plant and insect parasitic species showed from 5-12 MSP-hybridizing restriction fragments. Mammalian intestinal parasites showed between 1 and 13 bands hybridizing with the MSP probes. Blots probed to estimate the number of actin genes showed that, with the exception of Ascaris which contains more than 20 germ line sequences that encode actin, all of the nematodes tested had between 3 and 9 bands that hybridized to the Ascaris genomic actin probe. The possible use of highly conserved sequences such as MSP and actin to differentiate between nematode species in diagnostic and taxonomic studies is discussed. Copyright © Cambridge University Press 1989 major sperm protein nationallicence nematode actin nationallicence Ascaris nationallicence Caenorhabditis nationallicence cDNA sequences nationallicence Scott A. L. Department of Immunology and Infectious Diseases, School of Hygiene and Public Health, The Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD 21205 Dinman J. Department of Immunology and Infectious Diseases, School of Hygiene and Public Health, The Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD 21205 Sussman D. J. Department of Immunology and Infectious Diseases, School of Hygiene and Public Health, The Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD 21205 Ward S. Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210 Parasitology Cambridge University Press 98/3(1989-06), 471-478 0031-1820 98:3<471 1989 98 PAR https://doi.org/10.1017/S0031182000061564 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000061564 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Scott A. L. Department of Immunology and Infectious Diseases, School of Hygiene and Public Health, The Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD 21205 NATIONALLICENCE 700 1- Dinman J. Department of Immunology and Infectious Diseases, School of Hygiene and Public Health, The Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD 21205 NATIONALLICENCE 700 1- Sussman D. J. Department of Immunology and Infectious Diseases, School of Hygiene and Public Health, The Johns Hopkins University, 615 North Wolfe Street, Baltimore, MD 21205 NATIONALLICENCE 700 1- Ward S. Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210 NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/3(1989-06), 471-478 0031-1820 98:3<471 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge