caa a22 4500 38640061X CHVBK 20180307112157.0 cr unu---uuuuu 161130e198906 xx s 000 0 eng 10.1017/S0031182000061576 doi S0031182000061576 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000061576 Changes in nematode antigens recognized by monoclonal antibodies during early infections of soya beans with the cyst nematode Heterodera glycines [Elektronische Daten] The invasion and establishment of Heterodera glycines has been studied for 0-120 h after addition to host roots using a synchronized infection system. A high degree of synchrony in development was noted, with juvenile nematodes reaching their feeding sites adjacent to the root endodermis and discharging their subventral pharyngeal glands by 24 h post-invasion (p.i.). The moult to the 3rd-stage juvenile occurred approximately 89 h after entry to the root. Using an enzyme-linked immunosorbent assay with monoclonal antibodies (MAbs) whose tissue specificities had already been defined by immuno-cytochemistry, it was found that MAbs recognizing intestinal lipid droplets and granules showed a major increase in reactivity 18-24 h p.i. before declining by 39 h p.i. In contrast, the reactivity of two MAbs recognizing adult cuticle increased significantly only after 89 h p.i., following the second moult. MAbs to the two subventral pharyngeal gland cells showed a variety of patterns of changing reactivity during the experiment. One increased within 24 h of invasion before declining whereas a second fell without any initial rise. Two further MAbs specific for the subventral glands initially declined but showed a secondary increase in reactivity after 72 h and could be detected in adult females. This pattern was also seen in a MAb specific for the dorsal pharyngeal gland cell. These changes are discussed in the context of events taking place following invasion, with particular reference to the initiation and maintenance of a feeding syncytium by the developing nematode. Copyright © Cambridge University Press 1989 Heterodera glycines nationallicence nematode antigens nationallicence soya beans nationallicence invasion nationallicence establishment nationallicence Atkinson H. J. Department of Pure and Applied Biology, The University, Leeds LS2 9JT Harris P. D. Department of Pure and Applied Biology, The University, Leeds LS2 9JT Parasitology Cambridge University Press 98/3(1989-06), 479-487 0031-1820 98:3<479 1989 98 PAR https://doi.org/10.1017/S0031182000061576 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000061576 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Atkinson H. J. Department of Pure and Applied Biology, The University, Leeds LS2 9JT NATIONALLICENCE 700 1- Harris P. D. Department of Pure and Applied Biology, The University, Leeds LS2 9JT NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/3(1989-06), 479-487 0031-1820 98:3<479 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge