caa a22 4500 386400636 CHVBK 20180307112157.0 cr unu---uuuuu 161130e198906 xx s 000 0 eng 10.1017/S0031182000061461 doi S0031182000061461 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000061461 Cultivation of Blastocrithidia triatomae (Trypanosomatidae) on a cell line of its host Triatoma infestans (Reduviidae) [Elektronische Daten] Blastocrithidia triatomae parasitizes vectors of Chagas' disease and is very difficult to cultivate in conventional media. However, co-cultivation with a cell line of its host Triatoma infestans (TI-32; in Schneider's Drosophila medium supplemented with 20% foetal calf serum and 10% tryptose phosphate broth) led to vigorous growth at 24 or 28 °C without an adaptation phase. More than 60 primary cultures were initiated successfully without any failures. Subcultures could be started immediately at weekly intervals. The doubling time was similar in both the primary cultures (41 h) and the 33rd subculture (39 h). The importance of the reduviid cells for B. triatomae became clear after removal of the insect cells, when multiplication of epimastigotes stopped and mainly cysts were formed. Cysts produced in vitro were infective for reduviids. Scanning electron microscopy showed that B. triatomae attached to the host cells, inserted its flagellum into them and destroyed them. Copyright © Cambridge University Press 1989 Blastocrithidia triatomae nationallicence Chagas' disease nationallicence Triatoma infestans nationallicence Reduth Dagmar Institut für Biologie I (Zoologie), Albert-Ludwigs-Universität, Albertstrasse 21a, D-7800 Freiburg, Federal Republic of Germany Schaub G. A. Institut für Biologie I (Zoologie), Albert-Ludwigs-Universität, Albertstrasse 21a, D-7800 Freiburg, Federal Republic of Germany Pudney Mary Department of Entomology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, U.K. Parasitology Cambridge University Press 98/3(1989-06), 387-393 0031-1820 98:3<387 1989 98 PAR https://doi.org/10.1017/S0031182000061461 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000061461 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reduth Dagmar Institut für Biologie I (Zoologie), Albert-Ludwigs-Universität, Albertstrasse 21a, D-7800 Freiburg, Federal Republic of Germany NATIONALLICENCE 700 1- Schaub G. A. Institut für Biologie I (Zoologie), Albert-Ludwigs-Universität, Albertstrasse 21a, D-7800 Freiburg, Federal Republic of Germany NATIONALLICENCE 700 1- Pudney Mary Department of Entomology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, U.K NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/3(1989-06), 387-393 0031-1820 98:3<387 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge