caa a22 4500 388032707 CHVBK 20180307124956.0 cr unu---uuuuu 161130e199812 xx s 000 0 eng 10.1086/647766 doi S0195941700092158 pii (NATIONALLICENCE)cambridge-10.1086/647766 Epidemiological Analysis Defining Concurrent Outbreaks of Serratia marcescens and Methicillin-Resistant Staphylococcus aureus in a Neonatal Intensive-Care Unit [Elektronische Daten] Abstract Objective: To describe the epidemiology, interventions, and molecular typing methods used during the investigation and control of concurrent outbreaks of Serratia marcescens and methicillin-resistant Staphylococcus aureus (MRSA) infections in a neonatal intensive-care unit (NICU). Setting: A 206-bed women's and infants' hospital with a 48-bed NICU. Design: A 22-week, prospective, descriptive study of all NICU infants with S marcescens or MRSA infection or colonization. Repetitive polymerase chain reaction (rep PCR) and pulsed-field gel electrophoresis (PFGE), respectively, were applied to the typing of S marcescens and MRSA isolates. Interventions: Infants with S marcescens or MRSA infection or colonization were placed in isolation; all other infants were cohorted. A multidisciplinary task force implemented education for all hospital and medical staff regarding policies essential for outbreak control. Changes in physical setting and patient contact procedure were required to promote adherence to existing policies. Results: Two premature infants had S marcescens infection, and five were colonized; rep PCR verified that both invasive and three of five colonizing isolates were related genotypically. Five bacteremic and 10 MRSA-colonized infants were identified; PFGE confirmed that 12 of the isolates had similar electrophoretic patterns. S marcescens infection was eliminated from the NICU 3 weeks after interventions were initiated. MRSA infections also were eliminated, and MRSA colonization fell to below pre-outbreak rates within 8 weeks. Despite a 100% increase in NICU patient days per month during the subsequent 2 years, no further clusters of S marcescens or MRSA infection have occurred. Conclusions: Concurrent outbreaks of S marcescens and MRSA in an NICU were confirmed by genotyping of strains. Control was achieved by isolation and cohorting of patients and strict adherence to NICU policies and procedures. Copyright © The Society for Healthcare Epidemiology of America 1998 Campbell Judith R. Section of Infectious Diseases, Department of Pediatrics, Baylor College of Medicine Zaccaria Elena Department of Infection Control, Columbia Woman's Hospital of Texas, Houston, Texas Mason Edward O. Section of Infectious Diseases, Department of Pediatrics, Baylor College of Medicine Baker Carol J. Section of Infectious Diseases, Department of Pediatrics, Baylor College of Medicine Infection Control & Hospital Epidemiology Cambridge University Press 19/12(1998-12), 924-928 0899-823X 19:12<924 1998 19 ICE https://doi.org/10.1086/647766 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1086/647766 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Campbell Judith R. Section of Infectious Diseases, Department of Pediatrics, Baylor College of Medicine NATIONALLICENCE 700 1- Zaccaria Elena Department of Infection Control, Columbia Woman's Hospital of Texas, Houston, Texas NATIONALLICENCE 700 1- Mason Edward O. Section of Infectious Diseases, Department of Pediatrics, Baylor College of Medicine NATIONALLICENCE 700 1- Baker Carol J. Section of Infectious Diseases, Department of Pediatrics, Baylor College of Medicine NATIONALLICENCE 773 0- Infection Control & Hospital Epidemiology Cambridge University Press 19/12(1998-12), 924-928 0899-823X 19:12<924 1998 19 ICE CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge