caa a22 4500 388032928 CHVBK 20180307124957.0 cr unu---uuuuu 161130e199812 xx s 000 0 eng 10.1086/647764 doi S0195941700000898 pii (NATIONALLICENCE)cambridge-10.1086/647764 Detection of Bordetella pertussis and Respiratory Syncytial Virus in Air Samples From Hospital Rooms [Elektronische Daten] Abstract Objective: To evaluate the distribution of Bordetella pertussis and respiratory syncytial virus (RSV) in the hospital setting. Design: Air samples were collected using filters in the hospital rooms of 12 children with pertussis and 27 children with RSV infection. Material eluted from these filters was subjected to RSV- and B pertussis-specific polymerase chain reaction (PCR) amplification. Setting: Patients were hospitalized in private rooms in one of two referral centers, a university teaching hospital and a university-affiliated private children's hospital. Patients: 12 children (16 days-3 years of age) with documented pertussis infection and 27 patients (10 days-7 years of age) with documented RSV infection. Results: B pertussis DNA was detected in 7 (58%) of 12 rooms housing pertussis patients and in 16 (25%) of 63 total samples. B pertussis DNA was detected as far as 4 m away from the patient's bedside. The detection of B pertussis DNA in air samples did not change over the short duration of hospitalization. RSV RNA was detected in 17 (63%) of 27 rooms housing RSV-infected patients and in 32 (22%) of 143 total samples. RSV RNA was detected at distances as far as 7 m from the patient's bedside and for up to 7 days of hospitalization. Conclusions: Using PCR-based detection methods, B pertussis DNA and RSV RNA both can be detected in air samples from the hospital rooms of infected patients. Both can be detected at large distances from a patient's bedside in a minority of cases. These detection methods are suitable for further studies of control measures used to contain nosocomial infections caused by both B pertussis and RSV. Copyright © The Society for Healthcare Epidemiology of America 1998 Aintablian Nectar University of California, Children's Hospital and Health Center, San Diego, California Walpita Pramila University of California, Children's Hospital and Health Center, San Diego, California Sawyer Mark H. University of California, Children's Hospital and Health Center, San Diego, California Infection Control & Hospital Epidemiology Cambridge University Press 19/12(1998-12), 918-923 0899-823X 19:12<918 1998 19 ICE https://doi.org/10.1086/647764 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1086/647764 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aintablian Nectar University of California, Children's Hospital and Health Center, San Diego, California NATIONALLICENCE 700 1- Walpita Pramila University of California, Children's Hospital and Health Center, San Diego, California NATIONALLICENCE 700 1- Sawyer Mark H. University of California, Children's Hospital and Health Center, San Diego, California NATIONALLICENCE 773 0- Infection Control & Hospital Epidemiology Cambridge University Press 19/12(1998-12), 918-923 0899-823X 19:12<918 1998 19 ICE CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge