caa a22 4500 388033185 CHVBK 20180307124957.0 cr unu---uuuuu 161130e199805 xx s 000 0 eng 10.1086/647822 doi S0195941700087592 pii (NATIONALLICENCE)cambridge-10.1086/647822 Stability and Bactericidal Activity of Chlorine Solutions [Elektronische Daten] OBJECTIVES: To determine the stability of sodium hypochlorite (diluted household bleach) when stored for 30 days in various types of containers and to determine the efficacy of low concentrations of free available chlorine to inactivate test bacteria. DESIGN: Laboratory-based study. Solutions of standard household bleach were prepared using tap water or sterile distilled water at dilutions of 1:100, 1:50, and 1:5. Chlorine concentrations were measured, and then the solutions were placed into five polyethylene containers and left at room temperature (20°C) under various conditions (translucent containers with light exposure and with or without air; brown opaque container without light or air exposure). Samples for chlorine and pH determinations were taken at time 0 and on days 7, 14, 21, 30, and 40. Bactericidal activity of chlorine solutions was assessed using the Association of Official Analytical Chemists Use-Dilution Method. Test bacteria included Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella choleraesuis. RESULTS: Chlorine concentrations at 30 days varied from the 40% to 50% range for 1:50 or 1:100 dilutions stored in containers other than closed brown containers to 83% to 85% for the 1:5 dilution stored in closed but non-opaque containers to 97% to 100% for 1:50 or 1:5 solutions stored in closed brown containers. The lowest concentration of sodium hypochlorite solution that reliably inactivated all the test organisms was 100 ppm. CONCLUSIONS: These data suggest that chlorine solutions do not need to be prepared fresh daily, as is recommended currently, and the lowest concentration of chlorine that reliably inactivates S aureus, S choleraesuis, and P aeruginosa is 100 ppm Copyright © The Society for Healthcare Epidemiology of America 1998 Rutala William A. Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, North Carolina Cole Eugene C. Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, North Carolina Thomann Charlotte A. Department of Hospital Epidemiology, UNC Hospitals, Chapel Hill, North Carolina Weber David J. Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, North Carolina Infection Control & Hospital Epidemiology Cambridge University Press 19/5(1998-05), 323-327 0899-823X 19:5<323 1998 19 ICE https://doi.org/10.1086/647822 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1086/647822 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rutala William A. Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, North Carolina NATIONALLICENCE 700 1- Cole Eugene C. Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, North Carolina NATIONALLICENCE 700 1- Thomann Charlotte A. Department of Hospital Epidemiology, UNC Hospitals, Chapel Hill, North Carolina NATIONALLICENCE 700 1- Weber David J. Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, North Carolina NATIONALLICENCE 773 0- Infection Control & Hospital Epidemiology Cambridge University Press 19/5(1998-05), 323-327 0899-823X 19:5<323 1998 19 ICE CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge