caa a22 4500 388034432 CHVBK 20180307125000.0 cr unu---uuuuu 161130e199807 xx s 000 0 eng 10.1086/501799 doi S0195941700088093 pii (NATIONALLICENCE)cambridge-10.1086/501799 Cross-Contamination with Mycobacterium tuberculosis: An Epidemiological and Laboratory Investigation [Elektronische Daten] Abstract OBJECTIVE: To investigate possible cross-contamination of laboratory specimens, as suggested by an increased incidence of newly diagnosed patients with tuberculosis, many of whom had all negative smears for acid-fast bacilli and only one positive Mycobacterium tuberculosis culture referred to as "negative smears, one positive” or NSOP. METHODS: Medical-record reviews were performed for all patients with NSOP results diagnosed at this facility within a 9-month period. Laboratory logbooks were reviewed for all isolates processed; DNA fingerprinting was performed on available isolates. RESULTS: Of 80 patients with NSOP results, 45 (56%) were found to have false-positive cultures resulting from laboratory contamination with H37Ra, an avirulent stock strain of Mycobacterium tuberculosis. CONCLUSION: Laboratory cross-contamination resulted in the false diagnosis of tuberculosis in at least 45 individuals. Use of the Mycobacteria Growth Indicator Tube may have contributed to these contamination incidents by detecting small numbers of contaminating mycobacteria that may not have been detected with less sensitive media. Copyright © The Society for Healthcare Epidemiology of America 1998 Nivin Beth Bureau of Tuberculosis Control, New York City Department of Health, New York, New York Fujiwara Paula I. Bureau of Tuberculosis Control, New York City Department of Health, New York, New York Hannifin John Bureau of Tuberculosis Control, New York City Department of Health, New York, New York Kreiswirth Barry N. Public Health Research Institute, Centers for Disease Control and Prevention, Atlanta, Georgia Infection Control & Hospital Epidemiology Cambridge University Press 19/7(1998-07), 500-503 0899-823X 19:7<500 1998 19 ICE https://doi.org/10.1086/501799 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1086/501799 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nivin Beth Bureau of Tuberculosis Control, New York City Department of Health, New York, New York NATIONALLICENCE 700 1- Fujiwara Paula I. Bureau of Tuberculosis Control, New York City Department of Health, New York, New York NATIONALLICENCE 700 1- Hannifin John Bureau of Tuberculosis Control, New York City Department of Health, New York, New York NATIONALLICENCE 700 1- Kreiswirth Barry N. Public Health Research Institute, Centers for Disease Control and Prevention, Atlanta, Georgia NATIONALLICENCE 773 0- Infection Control & Hospital Epidemiology Cambridge University Press 19/7(1998-07), 500-503 0899-823X 19:7<500 1998 19 ICE CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge