caa a22 4500 388034815 CHVBK 20180307125001.0 cr unu---uuuuu 161130e199802 xx s 000 0 eng 10.1086/647774 doi S019594170000028X pii (NATIONALLICENCE)cambridge-10.1086/647774 Mycobacterium tuberculosis Specimen Contamination Revisited: The Role of Laboratory Environmental Control in a Pseudo-outbreak [Elektronische Daten] Abstract Objective: To investigate suspected pseudo-outbreaks of Mycobacterium tuberculosis (MTB) during August 1994 and July 1995 among patients who did not have clinical findings consistent with tuberculosis. Design: Retrospective and prospective surveys of all clinical and laboratory data using standard epidemiological tools and DNA fingerprinting. Setting: A university-affiliated community hospital. Patients: Those with positive MTB cultures during periods when we noted that the number of MTB positive cultures greatly outnumbered the usual monthly average (retrospective analysis, 1994) and patients with positive MTB cultures without clinical findings consistent with tuberculosis (prospective survey, 1995). Results: Epidemiological and molecular studies revealed specimen cross-contamination in the laboratory due to a faulty exhaust hood. Improvement in laboratory ventilation and change of the implicated hood prevented further specimen contamination. Conclusions: The identification of positive MTB cultures from patients without clinical evidence of tuberculosis should be a signal to suspect laboratory contamination and implement control measures. These should include a thorough epidemiological investigation, DNA fingerprint analysis, and an environmental inspection. Copyright © The Society for Healthcare Epidemiology of America 1998 Segal-Maurer Sorana Division of Infectious Diseases, the Department of Medicine, New York City, New York Kreiswirth Barry N. Cornell University Medical College, Public Health Research Institute, Tuberculosis Center, New York City, New York Burns Janice M. Department of Nursing, New York City, New York Lavie Sol Department of Pathology, Microbiology Laboratory, New York City, New York Lim Mimi Department of Nursing, New York City, New York Urban Carl Division of Infectious Diseases, the Department of Medicine, New York City, New York Rahal James J. Division of Infectious Diseases, the Department of Medicine, New York City, New York Infection Control & Hospital Epidemiology Cambridge University Press 19/2(1998-02), 101-105 0899-823X 19:2<101 1998 19 ICE https://doi.org/10.1086/647774 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1086/647774 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Segal-Maurer Sorana Division of Infectious Diseases, the Department of Medicine, New York City, New York NATIONALLICENCE 700 1- Kreiswirth Barry N. Cornell University Medical College, Public Health Research Institute, Tuberculosis Center, New York City, New York NATIONALLICENCE 700 1- Burns Janice M. Department of Nursing, New York City, New York NATIONALLICENCE 700 1- Lavie Sol Department of Pathology, Microbiology Laboratory, New York City, New York NATIONALLICENCE 700 1- Lim Mimi Department of Nursing, New York City, New York NATIONALLICENCE 700 1- Urban Carl Division of Infectious Diseases, the Department of Medicine, New York City, New York NATIONALLICENCE 700 1- Rahal James J. Division of Infectious Diseases, the Department of Medicine, New York City, New York NATIONALLICENCE 773 0- Infection Control & Hospital Epidemiology Cambridge University Press 19/2(1998-02), 101-105 0899-823X 19:2<101 1998 19 ICE CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge