caa a22 4500 388035919 CHVBK 20180307125004.0 cr unu---uuuuu 161130e199812 xx s 000 0 eng 10.1017/S0007485300054250 doi S0007485300054250 pii (NATIONALLICENCE)cambridge-10.1017/S0007485300054250 Molecular markers in entomology [Elektronische Daten] A diverse range of novel molecular (DNA) markers are now available for entomological investigations. Both DNA and protein markers have revolutionized the biological sciences and have enhanced many fields of study, especially ecology. Relative to DNA markers, allozymes are cheap, often much quicker to isolate and develop, even from minute insects (aphids, thrips, parasitic wasps, etc.), and subsequently easy to use. They display single or multi-locus banding patterns of a generally easily interpretable Mendelian nature, and the statistics for their analysis are well established. DNA markers are also suitable for use with small amounts of insect material and can be used with stored, dry or old samples. They have an expanding range of applications, many involving intra- and interspecific discriminations. Like allozymes, they can be single or multilocus, whilst methods for their statistical analysis have recently been published. However, they can be considerably more expensive than allozymes, require more complex preparatory protocols, expensive equipment, may involve lengthy development procedures (e.g. isolating cloned oligonucleotides to develop primers to detect microsatellite regions) and some have complex multi-locus banding patterns which may be of a non-Mendelian nature (e.g. RAPDs, randomly amplified polymorphic DNA), and are in some cases, not easily repeatable. In this review, we hope to inform the general reader about the methodology and scope of the main molecular markers commonly in use, along with brief details of some other techniques which show great promise for entomological studies. Thereafter, we discuss their applications including suitability for particular studies, the methods used to load and run samples, subsequent band detection, band scoring and interpretation, the reliability of particular techniques, the issues of safety involved, cost effectiveness and the statistical analyses utilized. Copyright © Cambridge University Press 1998 Loxdale H.D. Entomology and Nematology Department, IACR-Rothamsted, Harpenden, Hertfordshire, AL5 2JQ, UK Lushai G. School of Biological Sciences, Biodiversity and Ecology Division, Southampton University, Basset Crescent East, Southampton, SO16 7PX, UK Bulletin of Entomological Research Cambridge University Press 88/6(1998-12), 577-600 0007-4853 88:6<577 1998 88 BER https://doi.org/10.1017/S0007485300054250 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0007485300054250 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Loxdale H.D. Entomology and Nematology Department, IACR-Rothamsted, Harpenden, Hertfordshire, AL5 2JQ, UK NATIONALLICENCE 700 1- Lushai G. School of Biological Sciences, Biodiversity and Ecology Division, Southampton University, Basset Crescent East, Southampton, SO16 7PX, UK NATIONALLICENCE 773 0- Bulletin of Entomological Research Cambridge University Press 88/6(1998-12), 577-600 0007-4853 88:6<577 1998 88 BER CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge