caa a22 4500 388035986 CHVBK 20180307125004.0 cr unu---uuuuu 161130e199806 xx s 000 0 eng 10.1017/S0007485300025955 doi S0007485300025955 pii (NATIONALLICENCE)cambridge-10.1017/S0007485300025955 Bacillus thuringiensis isolates from the Philippines: habitat distribution, δ-endotoxin diversity, and toxicity to rice stem borers (Lepidoptera: Pyralidae) [Elektronische Daten] Bacillus thuringiensis Berliner isolates were detected in 57% of 801 samples of rice grain dust, soil, rice field arthropods, and miscellaneous habitats (rice straw compost and mammal faeces) collected at 100 sites in the Philippines. The collection yielded 3950 isolates of B. thuringiensis (8.7 isolates/positive sample). Grain dust from rice mills was the richest source (63% of the samples were positive, with 10.2 isolates/positive sample), followed by rice field arthropods, soil, and miscellaneous habitats. Polyclonal antibodies to six δ-endotoxin groups (Cry1A, Cry1B, Cry1C, Cry1D, Cry1E, and Cry3A) were used in enzyme-linked immunosorbent assays (ELISA) to characterize the toxins produced by each isolate. Subsamples of isolates representing the diversity of isolate sources and δ-endotoxin profiles were bioassayed against the yellow stem borer, Scirpophaga incertulas (Walker) and striped stem borer, Chilo suppressalis (Walker). Eighteen isolates highly toxic to both species were selected for characterization of δ-endotoxin genes by polymerase chain reaction (PCR) with primers specific to 14 genes or gene subfamilies, and Western blotting with Cry2A antibodies. At least two novel δ-endotoxin genes, related to cry1B and cry1F, were detected by DNA sequencing of PCR products. Copyright © Cambridge University Press 1998 Theunis W. Entomology and Plant Pathology Division, International Rice Research Institute, PO Box 933, 1099 Manila, Philippines Aguda R.M. Entomology and Plant Pathology Division, International Rice Research Institute, PO Box 933, 1099 Manila, Philippines Cruz W.T. Entomology and Plant Pathology Division, International Rice Research Institute, PO Box 933, 1099 Manila, Philippines Decock C. Plant Genetic Systems, N.V., J. Plateaustraat 22, B-9000 Ghent, Belgium Peferoen M. Plant Genetic Systems, N.V., J. Plateaustraat 22, B-9000 Ghent, Belgium Lambert B. Plant Genetic Systems, N.V., J. Plateaustraat 22, B-9000 Ghent, Belgium Bottrell D.G. Entomology and Plant Pathology Division, International Rice Research Institute, PO Box 933, 1099 Manila, Philippines Gould F.L. Department of Entomology, North Carolina State University, Raleigh NC 27695, USA Litsinger J.A. Entomology and Plant Pathology Division, International Rice Research Institute, PO Box 933, 1099 Manila, Philippines Cohen M.B. Entomology and Plant Pathology Division, International Rice Research Institute, PO Box 933, 1099 Manila, Philippines Bulletin of Entomological Research Cambridge University Press 88/3(1998-06), 335-342 0007-4853 88:3<335 1998 88 BER https://doi.org/10.1017/S0007485300025955 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0007485300025955 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Theunis W. Entomology and Plant Pathology Division, International Rice Research Institute, PO Box 933, 1099 Manila, Philippines NATIONALLICENCE 700 1- Aguda R.M. Entomology and Plant Pathology Division, International Rice Research Institute, PO Box 933, 1099 Manila, Philippines NATIONALLICENCE 700 1- Cruz W.T. Entomology and Plant Pathology Division, International Rice Research Institute, PO Box 933, 1099 Manila, Philippines NATIONALLICENCE 700 1- Decock C. Plant Genetic Systems, N.V., J. Plateaustraat 22, B-9000 Ghent, Belgium NATIONALLICENCE 700 1- Peferoen M. Plant Genetic Systems, N.V., J. Plateaustraat 22, B-9000 Ghent, Belgium NATIONALLICENCE 700 1- Lambert B. Plant Genetic Systems, N.V., J. Plateaustraat 22, B-9000 Ghent, Belgium NATIONALLICENCE 700 1- Bottrell D.G. Entomology and Plant Pathology Division, International Rice Research Institute, PO Box 933, 1099 Manila, Philippines NATIONALLICENCE 700 1- Gould F.L. Department of Entomology, North Carolina State University, Raleigh NC 27695, USA NATIONALLICENCE 700 1- Litsinger J.A. Entomology and Plant Pathology Division, International Rice Research Institute, PO Box 933, 1099 Manila, Philippines NATIONALLICENCE 700 1- Cohen M.B. Entomology and Plant Pathology Division, International Rice Research Institute, PO Box 933, 1099 Manila, Philippines NATIONALLICENCE 773 0- Bulletin of Entomological Research Cambridge University Press 88/3(1998-06), 335-342 0007-4853 88:3<335 1998 88 BER CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge