caa a22 4500 388060425 CHVBK 20180307125119.0 cr unu---uuuuu 161130e199808 xx s 000 0 eng 10.1017/S1357729800009875 doi S1357729800009875 pii (NATIONALLICENCE)cambridge-10.1017/S1357729800009875 Influence of dietary protein of diets given in late gestation and during lactation on protein content and oestrogen concentrations in the colostrum and milk of gilts [Elektronische Daten] Forty first cross hybrid gilts were used to study the effect of dietary protein during lactation on free amino acid, protein and total oestrogen concentrations in colostrum and milk. The diets were formulated to contain similar levels of digestible energy (DE) but with either 236 or 186 g protein per kg and 13·3 and 8·1 g lysine per kg air-dried diet respectively. Both diets were offered at 2·5 kg/day for 1 week prior to farrowing and ad libitum throughout a 24-day lactation (no. = 20 gilts per treatment). Colostrum and milk were collected manually at 3h (colostrum), 3, 7, 14 and 22 days (milk) post partum. Free amino acid concentrations in colostrum and milk were determined by high-performance liquid chromatography following derivatization of the amino acids by 9-fluorenylmethyl chloroformate. The lysine and glutamic acid concentrations in colostrum were the most abundant free amino acids followed by tryptophan and taurine in order of decreasing abundance. Milk free taurine concentrations increased to the greatest extent among all free amino acids over the 21 days of lactation. Gilts' mature milk was rich in free taurine, glutamic acid, glycine and lysine. During the first 3 h post partum, the protein content of colostrum was unaffected by dietary protein. However, milk protein content over 7 to 21 days of lactation was significantly higher in gilts offered the higher protein diet (P < 0·001). Total solids in colostrum and milk was not significantly affected by dietary protein. At 3h and 3 days post partum the concentrations of total oestrogen in colostrum and milk, determined by radioimmunoassay, were significantly higher in gilts offered the high protein diet (P < 0·05). These finding indicate that a high protein diet in late gestation and during lactation can influence protein content, amino acid and oestrogen concentrations of milk in gilts. Copyright © British Society of Animal Science 1998 gilts nationallicence colostrum nationallicence milk composition nationallicence oestrogens nationallicence dietary protein nationallicence Al-Matubsi H. Y. Centre for Bioprocessing and Food Technology, Victoria University of Technology, Werribee Campus, PO Box 14428 MCMC Melbourne, Victoria 8001, Australia Bervanakis G. Centre for Bioprocessing and Food Technology, Victoria University of Technology, Werribee Campus, PO Box 14428 MCMC Melbourne, Victoria 8001, Australia Tritton S. M. Rhone Poulenc Animal Nutrition, West Footscray, Victoria 3012, Australia Campbell R. G. Bunge Meat Industries, PO Box 78, Corowa, NSW 2646, Australia Fairclough R. J. Department of Biomedical Sciences, Victoria University of Technology, St Albans Campus, PO Box 14428 MCMC Melbourne, Victoria 8001, Australia Animal Science Cambridge University Press 67/1(1998-08), 139-145 1357-7298 67:1<139 1998 67 ASC https://doi.org/10.1017/S1357729800009875 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S1357729800009875 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Al-Matubsi H. Y. Centre for Bioprocessing and Food Technology, Victoria University of Technology, Werribee Campus, PO Box 14428 MCMC Melbourne, Victoria 8001, Australia NATIONALLICENCE 700 1- Bervanakis G. Centre for Bioprocessing and Food Technology, Victoria University of Technology, Werribee Campus, PO Box 14428 MCMC Melbourne, Victoria 8001, Australia NATIONALLICENCE 700 1- Tritton S. M. Rhone Poulenc Animal Nutrition, West Footscray, Victoria 3012, Australia NATIONALLICENCE 700 1- Campbell R. G. Bunge Meat Industries, PO Box 78, Corowa, NSW 2646, Australia NATIONALLICENCE 700 1- Fairclough R. J. Department of Biomedical Sciences, Victoria University of Technology, St Albans Campus, PO Box 14428 MCMC Melbourne, Victoria 8001, Australia NATIONALLICENCE 773 0- Animal Science Cambridge University Press 67/1(1998-08), 139-145 1357-7298 67:1<139 1998 67 ASC CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge