caa a22 4500 388060514 CHVBK 20180307125119.0 cr unu---uuuuu 161130e199808 xx s 000 0 eng 10.1017/S1357729800009899 doi S1357729800009899 pii (NATIONALLICENCE)cambridge-10.1017/S1357729800009899 Effect of method of blood sample collection on adrenal activity in farmed red deer and sheep following administration of ACTH [Elektronische Daten] The effect of method of blood sample collection (automatic blood sampling equipment (ABSE) v. manual) on cortisol and progesterone concentrations was investigated in 20 farmed red deer hinds and 20 domestic sheep ewes following dexamethasone and exogenous ACTH administration. Ten animals were subjected to either automatic sampling or manual sampling via jugular venipuncture in 1 week, with the treatment groups reversed in the 2nd week. The ABSE was programmed to collect a blood sample, then deliver 2 mg dexamethasone, collect a further blood sample 120 min later and then inject 100 fig ACTH. Thereafter, samples were collected at 15-min intervals during a 2·5 h period (12 samples in total). In the manual injection and sampling treatment, four samples were collected: (1) before dexamethasone administration, (2) before ACTH administration, (3) 60 min after ACTH administration, and (4) 150 min after ACTH administration. The success rate of blood sampling with ABSE was 80%. The overall mean packed cell volume (PCV) from samples collected by ABSE from both hinds and ewes was significantly lower than that from samples collected manually (P < 0·01) and PCV declined with time in manually sampled animals (P < 0·01). Plasma cortisol concentrations peaked at 45 min after ACTH administration in sheep and deer. In sheep, there was a marked fluctuation in the plasma cortisol concentrations with time. Both deer and sheep showed a reduced cortisol response to ACTH during week 2 irrespective of sampling method suggesting down-regulation of the response to ACTH. Maximum mean plasma progesterone concentration was reached at 15 to 30 min after ACTH administration. No significant differences in cortisol and progesterone responses due to blood sampling method were found in animals receiving prior dexamethasone treatment. This demonstrates that the ABSE has the ability to be used to effectively conduct ACTH stimulation tests without the need to handle the animals during the test. Copyright © British Society of Animal Science 1998 blood sampling nationallicence deer nationallicence hydrocortisone nationallicence progesterone nationallicence sheep nationallicence Ferre I. Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH Goddard P. J. Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH Macdonald A. J. Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH Littlewood C. A. Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH Duff E. I. Biomathematics and Statistics Scotland, Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH Animal Science Cambridge University Press 67/1(1998-08), 157-164 1357-7298 67:1<157 1998 67 ASC https://doi.org/10.1017/S1357729800009899 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S1357729800009899 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ferre I. Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH NATIONALLICENCE 700 1- Goddard P. J. Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH NATIONALLICENCE 700 1- Macdonald A. J. Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH NATIONALLICENCE 700 1- Littlewood C. A. Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH NATIONALLICENCE 700 1- Duff E. I. Biomathematics and Statistics Scotland, Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH NATIONALLICENCE 773 0- Animal Science Cambridge University Press 67/1(1998-08), 157-164 1357-7298 67:1<157 1998 67 ASC CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge