caa a22 4500 388061340 CHVBK 20180307125121.0 cr unu---uuuuu 161130e199802 xx s 000 0 eng 10.1017/S1357729800008869 doi S1357729800008869 pii (NATIONALLICENCE)cambridge-10.1017/S1357729800008869 Patterns of oestrus, time of LH release and ovulation and effects of time of artificial insemination in Mediterranean buffalo cows [Elektronische Daten] Thirty-two post-partum Mediterranean river buffalo cows were continuously observed for signs of oestrus from September to December with the aid of two vasectomized bulls. Symptoms of oestrus among female Mediterranean buffaloes are weak, therefore oestrus was assessed based on bull behaviour (following and licking a cow and trying to mount her). Oestrus was divided into three phases based on the bull behaviour assessment. Status of the uterus and ovarian follicles were checked rectally every day for each cow which was detected by a teaser bull to be in oestrus. All cows in oestrus were bred twice by artificial insemination (AI), the first at ovulation and the second (using a different bull) 22 h later. The average duration of interest shown by the bull towards a cow (from the very first to the last sign of interest) was 68 h and the average length of the three phases was: phase 1 = 21 (s.d. 29) h, phase II = 32 (s.d. 24) h and phase III = 15 (s.d. 15) h. Fifteen buffaloes were bled during oestrus, and LH profiles were determined. No differences were evident among oestruses followed by pregnancy (no. = 18) and the others (no. = 26) for the variables describing behavioural events. Neither intensity of the bull courtship, presence or clarity of mucous discharge, or housing system affected the success of AI. The only differences between pregnant and non-pregnant cows were in the timing between the LH peak and the end of phase II (2·4 v. 14·7 h, P < 0·001), end of phase III (22 v. 40 h, P > 0·05) and ovulation (25 v. 46 h, P < 0·05). Successful pregnancies occurred 34 (s.d. 14) h after the end of phase II. The endocrinology and behavioural patterns of buffalo reproduction need further research to clarify the reasons for non-optimal pregnancy rates after AI. Copyright © British Society of Animal Science 1998 artificial insemination nationallicence buffaloes nationallicence LH nationallicence oestrus nationallicence Moioli B. M. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy Napolitano F. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy Puppo S. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy Barile V. L. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy Terzano G. M. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy Borghese A. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy Malfatti A. Istituto di Fisiologia, Facoltá di Veterinaria, via San Costanzo 4, 06126 Perugia, Italy Catalano A. Istituto di Fisiologia, Facoltá di Veterinaria, via San Costanzo 4, 06126 Perugia, Italy Pilla A. M. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy Animal Science Cambridge University Press 66/1(1998-02), 87-91 1357-7298 66:1<87 1998 66 ASC https://doi.org/10.1017/S1357729800008869 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S1357729800008869 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moioli B. M. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy NATIONALLICENCE 700 1- Napolitano F. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy NATIONALLICENCE 700 1- Puppo S. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy NATIONALLICENCE 700 1- Barile V. L. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy NATIONALLICENCE 700 1- Terzano G. M. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy NATIONALLICENCE 700 1- Borghese A. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy NATIONALLICENCE 700 1- Malfatti A. Istituto di Fisiologia, Facoltá di Veterinaria, via San Costanzo 4, 06126 Perugia, Italy NATIONALLICENCE 700 1- Catalano A. Istituto di Fisiologia, Facoltá di Veterinaria, via San Costanzo 4, 06126 Perugia, Italy NATIONALLICENCE 700 1- Pilla A. M. Istituto Sperimentale per la Zootecnia, via Salaria 31, 00016 Monterotondo, Italy NATIONALLICENCE 773 0- Animal Science Cambridge University Press 66/1(1998-02), 87-91 1357-7298 66:1<87 1998 66 ASC CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge