caa a22 4500 388062096 CHVBK 20180307125124.0 cr unu---uuuuu 161130e199803 xx s 000 0 eng 10.1079/BJN19980045 doi S0007114598000464 pii (NATIONALLICENCE)cambridge-10.1079/BJN19980045 The interaction between nutritional status and growth hormone in young cattle: differential responsiveness of fat and protein metabolism [Elektronische Daten] The effect of dietary intake level on in vivo plasma leucine and plasma palmitate flux rates and on the response to a bolus injection of bovine growth hormone (GH) was investigated in six young steers. Animals were fed on a pelleted diet of dried grass-barley (0.7:0.3, w/w) in quantities sufficient to supply 0.8, 1.2, 1.6, 2.0, 2.4 or 2.65 × maintenance energy requirement, offered in hourly portions. Continuous intravenous infusions of [1-13C]leucine or [1-13C]palmi-tate were used to determine the flux of amino acid and fatty acid through the plasma pool before, immediately (1-3 h) after and 22-24 h after a subcutaneous injection of bovine GH (0.55 mg/kg body weight). Hourly blood samples were taken for 27 h to monitor the temporal responses of circulating hormones and metabolites following GH administration. The animal on the lowest plane of nutrition had elevated plasma GH and reduced insulin-like growth factor-1 concentrations compared with those fed on higher intake levels. Plasma leucine flux and leucine concentration increased with intake while palmitate flux and plasma non-esterified fatty acid (NEFA) concentrations were inversely related to intake. Leucine flux rate decreased in the animals fed on the two highest intake levels in response to GH 22-24 h after administration, but plasma leucine concentrations were reduced in all animals at this time. Only the animal fed on the lowest intake level showed an immediate response to GH (within 3 h of administration) with increased palmitate flux and plasma NEFA concentrations but a lipolytic response was apparent in other animals 22-24 h post-administration although the magnitude of the response was markedly reduced at high intakes. We conclude that lipid and protein metabolism are differentially responsive to GH and nutritional status. Copyright © The Nutrition Society 1998 Growth hormone nationallicence Palmitate nationallicence Leucine nationallicence Cattle nationallicence Dawson Janet M. School of Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK Greathead Henry M. R. School of Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK Craigon Jim School of Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK Hachey David L. USDA Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA Reeds Peter J. USDA Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA Pell Jennifer M. Babraham Institute, Babraham, Cambridge CB2 4AT, UK Buttery Peter J. School of Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK British Journal of Nutrition Cambridge University Press 79/3(1998-03), 275-286 0007-1145 79:3<275 1998 79 BJN https://doi.org/10.1079/BJN19980045 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19980045 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dawson Janet M. School of Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK NATIONALLICENCE 700 1- Greathead Henry M. R. School of Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK NATIONALLICENCE 700 1- Craigon Jim School of Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK NATIONALLICENCE 700 1- Hachey David L. USDA Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA NATIONALLICENCE 700 1- Reeds Peter J. USDA Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA NATIONALLICENCE 700 1- Pell Jennifer M. Babraham Institute, Babraham, Cambridge CB2 4AT, UK NATIONALLICENCE 700 1- Buttery Peter J. School of Biology, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 79/3(1998-03), 275-286 0007-1145 79:3<275 1998 79 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge