caa a22 4500 388062207 CHVBK 20180307125124.0 cr unu---uuuuu 161130e199803 xx s 000 0 eng 10.1079/BJN19980040 doi S0007114598000415 pii (NATIONALLICENCE)cambridge-10.1079/BJN19980040 Excretion of endogenous and exogenous purine derivatives in sheep: effect of increased concentrate intake [Elektronische Daten] The present study examined the endogenous urinary excretion of purine derivatives (PD; allantoin, uric acid and xanthine plus hypoxanthine) in fed animals. Four Rasa Aragonesa ewes fitted with simple cannulas in the rumen and proximal duodenum were used. Animals were given a lucerne (Medicago sativa) hay diet, as sole feed (A) or supplemented, respectively, with 220 (B), 400 (C), and 550 (D) g rolled barley grain/d following a 4 × 4 random factorial design. Duodenal flow of purine bases (PB) was determined by the dual-phase marker system. 15N was infused continuously into the rumen to label exogenous or microbial PB. Duodenal PB flow and urinary excretion of PD increased with digestible organic matter intake showing a constant recovery of duodenal PB. The isotope dilution of PD in urine samples confirmed the presence of an endogenous fraction, originating from tissues, that increased from 115.2 (SE 5.84) μmol/kg W0.75 for the basal diet to 304.2 (SE 7.6) μmol/kg W0.75 at the highest level of duodenal PB. Copyright © The Nutrition Society 1998 Purines nationallicence Feed intake nationallicence Sheep nationallicence Pérez J. F. Departamento Producción Animal y Ciencia de los Alimentos Biología Molecular y Celular, Facultad de Veterinaria, Miguel Servet 177, 50013 Zaragoza, Spain Balcells J. Departamento Producción Animal y Ciencia de los Alimentos Biología Molecular y Celular, Facultad de Veterinaria, Miguel Servet 177, 50013 Zaragoza, Spain Cebrián J. A. Departamento de Bioquímica, Biología Molecular y Celular, Facultad de Veterinaria, Miguel Servet 177, 50013 Zaragoza, Spain Martín-Orúe S. M. Departamento Producción Animal y Ciencia de los Alimentos Biología Molecular y Celular, Facultad de Veterinaria, Miguel Servet 177, 50013 Zaragoza, Spain British Journal of Nutrition Cambridge University Press 79/3(1998-03), 237-240 0007-1145 79:3<237 1998 79 BJN https://doi.org/10.1079/BJN19980040 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19980040 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pérez J. F. Departamento Producción Animal y Ciencia de los Alimentos Biología Molecular y Celular, Facultad de Veterinaria, Miguel Servet 177, 50013 Zaragoza, Spain NATIONALLICENCE 700 1- Balcells J. Departamento Producción Animal y Ciencia de los Alimentos Biología Molecular y Celular, Facultad de Veterinaria, Miguel Servet 177, 50013 Zaragoza, Spain NATIONALLICENCE 700 1- Cebrián J. A. Departamento de Bioquímica, Biología Molecular y Celular, Facultad de Veterinaria, Miguel Servet 177, 50013 Zaragoza, Spain NATIONALLICENCE 700 1- Martín-Orúe S. M. Departamento Producción Animal y Ciencia de los Alimentos Biología Molecular y Celular, Facultad de Veterinaria, Miguel Servet 177, 50013 Zaragoza, Spain NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 79/3(1998-03), 237-240 0007-1145 79:3<237 1998 79 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge