caa a22 4500 388062304 CHVBK 20180307125124.0 cr unu---uuuuu 161130e199804 xx s 000 0 eng 10.1079/BJN19980063 doi S0007114598000646 pii (NATIONALLICENCE)cambridge-10.1079/BJN19980063 Metabolism of parenterally administered fat emulsions in the rat: studies of fatty acid oxidation with 1-13C- and 8-13C-labelled triolein [Elektronische Daten] To reassess the hypothesis that fatty acid catabolism occurs to completion via β-oxidation, male Sprague-Dawley rats receiving continuous total parenteral nutrition (TPN) including 43% energy as fat were infused with [1-13C]- or [8-13C]triolein. Expired CO2 was collected continuously for 4 h and its 13C: 12C ratio determined by isotope-ratio mass spectrometry. Bicarbonate retention was also assessed over 4 h by infusion of NaH14CO3 and measurement of the expired 14CO2. A possible loss of label from [8-13C]oleic acid from the citric acid cycle via labelled acetyl-CoA without oxidation to CO2 was assessed by infusing further animals with acetate labelled with 14C either at C atoms 1 or 2 and determination of its conversion to expired 14CO2. At isotopic steady state, 63.2 (SE 1.6)% (n 8) of the infused [1-14C]acetate and 46.0 (SE 1.2)% (n 8) of [2-14C]acetate was recovered as expired 14CO2. After correction for bicarbonate retention and non-oxidative isotope loss, 37.3 (SE 1.2)% (n 20) of the [1-13C]triolein was found to have been oxidized, whereas 32.6 (SE 1.0)% (n 20) of the [8-13C]triolein was oxidized (P ≤ 0.01). The lower oxidation of the C atom at position 8 of oleic acid than that at position 1 indicates incomplete oxidative breakdown of the fatty acid after entering β-oxidation. Copyright © The Nutrition Society 1998 13C-labelled triolein nationallicence β-oxidation nationallicence Total parenteral nutrition nationallicence Bäurle Wolfgang Institute for Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany Brösicke Herbert GCMS-Laboratory, Children's Hospital of the Free University of Berlin, Heubnerweg 16, 14059 Berlin, Germany Matthews Dwight E. Department of Medicine, The University of Vermont College of Medicine, Given Building B217, Endo, Burlington, VT 05405, USA Pogan Karin Institute for Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany Fürst Peter Institute for Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany British Journal of Nutrition Cambridge University Press 79/4(1998-04), 381-387 0007-1145 79:4<381 1998 79 BJN https://doi.org/10.1079/BJN19980063 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19980063 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bäurle Wolfgang Institute for Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany NATIONALLICENCE 700 1- Brösicke Herbert GCMS-Laboratory, Children's Hospital of the Free University of Berlin, Heubnerweg 16, 14059 Berlin, Germany NATIONALLICENCE 700 1- Matthews Dwight E. Department of Medicine, The University of Vermont College of Medicine, Given Building B217, Endo, Burlington, VT 05405, USA NATIONALLICENCE 700 1- Pogan Karin Institute for Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany NATIONALLICENCE 700 1- Fürst Peter Institute for Biological Chemistry and Nutrition, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 79/4(1998-04), 381-387 0007-1145 79:4<381 1998 79 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge