caa a22 4500 388062487 CHVBK 20180307125125.0 cr unu---uuuuu 161130e199806 xx s 000 0 eng 10.1079/BJN19980091 doi S0007114598000920 pii (NATIONALLICENCE)cambridge-10.1079/BJN19980091 Rat adipose tissue rapidly accumulates and slowly releases an orally-administered high vitamin D dose [Elektronische Daten] We investigated the effect of oral high-dose cholecalciferol on plasma and adipose tissue cholecalciferol and its subsequent release, and on plasma 25-hydroxyvitamin D (25(OH)D). Female Wistar rats (n 126) received 37·5 μg cholecalciferol/d for 14 d and were subsequently studied for a further 88 d. Two subgroups of eighteen rats each were fasted for 3 d immediately after treatment (days 14−17) and at the end of the study (days 98−101). During treatment, plasma cholecalciferol increased rapidly to reach a steady-state. Plasma 25(OH)D and adipose tissue cholecalciferol increased linearly for 1 - 2 d after treatment. Serum Ca and inorganic phosphate also increased. Subsequently half-lives of plasma cholecalciferol and 25(OH)D, and perirenal and subcutaneous adipose tissue were: 1·4, 22·5, 97·5 and 80·9 d respectively. Fasting, as compared with ad libitum feeding, caused increased plasma free fatty acids, weight loss up to 14% and increased adipose tissue cholecalciferol (nmol/g wet weight). It did not affect plasma cholecalciferol immediately after cholecalciferol treatment, but raised plasma 25(OH)D. Fasting at the end of the study decreased plasma cholecalciferol and increased plasma 25(OH)D. We conclude that orally-administered cholecalciferol rapidly accumulates in adipose tissue and that it is very slowly released while there is energy balance. Fasting causes preferential loss of triacylglycerols from adipose tissue, as opposed to cholecalciferol, but nevertheless augments plasma 25(OH)D. Adipose tissue may act as a ‘buffer to functional vitamin D status' by preventing, to a certain extent, unregulated production of 25(OH)D from dietary vitamin D, and by slowly releasing vitamin D under fasting conditions. Copyright © The Nutrition Society 1998 Cholecalciferol nationallicence Plasma 25-hydroxyvitamin D nationallicence Calcium nationallicence Blood nationallicence Brouwer D. A. Janneke Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands Beek Jackelieng Van Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands Ferwerda Harri Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands Brugman Astrid M. Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands Klis Fiona R. M. van der Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands Heiden H. Jacoline van der Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands Muskiet Frits A. J. Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands British Journal of Nutrition Cambridge University Press 79/6(1998-06), 527-532 0007-1145 79:6<527 1998 79 BJN https://doi.org/10.1079/BJN19980091 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19980091 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brouwer D. A. Janneke Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands NATIONALLICENCE 700 1- Beek Jackelieng Van Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands NATIONALLICENCE 700 1- Ferwerda Harri Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands NATIONALLICENCE 700 1- Brugman Astrid M. Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands NATIONALLICENCE 700 1- Klis Fiona R. M. van der Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands NATIONALLICENCE 700 1- Heiden H. Jacoline van der Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands NATIONALLICENCE 700 1- Muskiet Frits A. J. Central Laboratory for Clinical Chemistry, University Hospital Groningen, PO Box 30.001, 97 RB Groningen, The Netherlands NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 79/6(1998-06), 527-532 0007-1145 79:6<527 1998 79 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge