caa a22 4500 388062576 CHVBK 20180307125125.0 cr unu---uuuuu 161130e199812 xx s 000 0 eng 10.1017/S0007114598001676 doi S0007114598001676 pii (NATIONALLICENCE)cambridge-10.1017/S0007114598001676 Short-term ingestion of chlorogenic or caffeic acids decreases zinc but not copper absorption in rats, utilization of stable isotopes and inductively-coupled plasma mass spectrometry technique [Elektronische Daten] The amount of dietary trace elements absorbed from a meal depends, among other factors, on the quantities of certain minor plant constituents present in the meal. These substances can act as ligands and bind trace elements in the digestive tract in available or unavailable forms for absorption. The present study was designed to investigate the extent to which different polyphenols (PP) may influence Zn and Cu absorption in rats. Different PP of nutritional interest (chlorogenic acid, caffeic acid, catechin and rutin) were studied using meals extrinsically-labelled with stable isotopes67Zn and 65Cu. Male Wistar rats were fed on a non-labelled semi-synthetic diet containing (mg/kg) 38 Fe, 35 Zn and 7·5 Cu for 8 d. PP were dissolved in dimethyl sulfoxide as the solvent and added to the meal at 1 g/kg during 3 d before isotope administration and until the end of the experiment (a further 3 d). The control group received the dimethyl sulfoxide only. After overnight food deprivation, rats were fed on the labelled test meals (4 g diet+0·1 mg 67Zn and 0·1 mg 65Cu) with 0·5 mg Dy as a faecal marker. Faeces and urine pools were collected for 3 d and analysed for 67Zn and65 Cu isotopic enrichment using the inductively-coupled plasma mass spectrometry technique. Zn absorption was significantly less in rats fed on chlorogenic acid or caffeic acid than in the control group. Catechin ingestion non-significantly inhibited 67Zn absorption. However, the PP studied were without effect on Cu absorption. The study illustrates the effect of metal-binding phenolic compounds on mineral nutrition in the rat, and the possible importance of the effects of different foods rich in these compounds on mineral absorption in man. Copyright © The Nutrition Society 1998 Polyphenols nationallicence Cu absorption nationallicence Zn absorption nationallicence Isotope technique nationallicence Coudray Charles Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, France Bousset Carole Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, France Tressol Jean C. Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, France Pépin Denise Laboratoire d'hydrologie, Institut Louise Blanquet, Faculté de Pharmacie, Université d'Auvergne, Clermont-Ferrand, France Rayssiguier Yves Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, France British Journal of Nutrition Cambridge University Press 80/6(1998-12), 575-584 0007-1145 80:6<575 1998 80 BJN https://doi.org/10.1017/S0007114598001676 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0007114598001676 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Coudray Charles Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, France NATIONALLICENCE 700 1- Bousset Carole Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, France NATIONALLICENCE 700 1- Tressol Jean C. Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, France NATIONALLICENCE 700 1- Pépin Denise Laboratoire d'hydrologie, Institut Louise Blanquet, Faculté de Pharmacie, Université d'Auvergne, Clermont-Ferrand, France NATIONALLICENCE 700 1- Rayssiguier Yves Centre de Recherche en Nutrition Humaine d'Auvergne, Laboratoire Maladies Métaboliques et Micronutriments, INRA de Clermont-Ferrand/Theix, 63122 Saint Genès Champanelle, France NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 80/6(1998-12), 575-584 0007-1145 80:6<575 1998 80 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge