caa a22 4500 388062649 CHVBK 20180307125125.0 cr unu---uuuuu 161130e199805 xx s 000 0 eng 10.1079/BJN19980075 doi S0007114598000762 pii (NATIONALLICENCE)cambridge-10.1079/BJN19980075 Effect of protein restriction on messenger RNA of insulin-like growth factor-I and insulin-like growth factor-binding proteins in liver of ovariectomized rats [Elektronische Daten] Effects of dietary protein restriction and ovariectomy on plasma concentrations and hepatic messenger RNA (mRNA) of insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding proteins (IGFBP) were investigated in young female rats. Ovariectomy increased plasma IGF-I concentration in rats fed on either a 50 g casein/kg diet (protein-restricted diet) or a 200 g casein/kg diet (control diet), but it increased IGF-I mRNA in liver only in the rats fed on the control diet. On the other hand, by Western ligand blot analysis, we observed that ovariectomy increased plasma IGFBP-3 concentration, and decreased plasma IGFBP-4 concentration. Ovariectomy did not affect IGFBP-1 and IGFBP-2 mRNA in liver, but dietary protein restriction significantly increased them, which may correspond to their plasma concentrations. The present results show that ovarian hormones and dietary protein content affect the plasma concentrations of IGF-I, IGFBP-3 and IGFBP-4 and hepatic mRNA of IGF-I, IGFBP-1, IGFBP-2, IGFBP-3 and IGFBP-4 in different manners. Copyright © The Nutrition Society 1998 Insulin-like growth factor-I nationallicence Insulin-like growth factor-binding proteins nationallicence Protein restriction nationallicence Ovariectomy nationallicence Higashi Yusuke Laboratory of Nutritional Biochemistry, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyoku, Tokyo 113, Japan Takenaka Asako Laboratory of Nutritional Biochemistry, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyoku, Tokyo 113, Japan Takahashi Shin-Ichiro Laboratory of Nutritional Biochemistry, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyoku, Tokyo 113, Japan Noguchi Tadashi Laboratory of Nutritional Biochemistry, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyoku, Tokyo 113, Japan British Journal of Nutrition Cambridge University Press 79/5(1998-05), 447-453 0007-1145 79:5<447 1998 79 BJN https://doi.org/10.1079/BJN19980075 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19980075 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Higashi Yusuke Laboratory of Nutritional Biochemistry, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyoku, Tokyo 113, Japan NATIONALLICENCE 700 1- Takenaka Asako Laboratory of Nutritional Biochemistry, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyoku, Tokyo 113, Japan NATIONALLICENCE 700 1- Takahashi Shin-Ichiro Laboratory of Nutritional Biochemistry, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyoku, Tokyo 113, Japan NATIONALLICENCE 700 1- Noguchi Tadashi Laboratory of Nutritional Biochemistry, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyoku, Tokyo 113, Japan NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 79/5(1998-05), 447-453 0007-1145 79:5<447 1998 79 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence SWISSBIB 387773355 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge