caa a22 4500 38806305X CHVBK 20180307125126.0 cr unu---uuuuu 161130e199809 xx s 000 0 eng 10.1017/S0007114598001317 doi S0007114598001317 pii (NATIONALLICENCE)cambridge-10.1017/S0007114598001317 Estimation of energy expenditure in free-living red deer (Cervus elaphus) with the doubly-labelled water method [Elektronische Daten] Energy expenditure was estimated using the doubly-labelled water (DLW) method in summer in five free-living adult, non-pregnant, non-lactating, red deer (Cervus elaphus) hinds (weight 107.3 (se 0.9) kg; age 6 (se 1) years) on lowland pasture under typical farming conditions. Climatic conditions were monitored throughout the experiment. Errors due to 2H losses in CH4 and faeces were calculated from previous estimates of stoichiometries. CH4 production, fractionated water loss, urinary N and O2 consumption were estimated using an iterative approach. The water flux (rH2O) in these animals consuming only fresh grass was 12 (se 0.5) kg/d, the CO2 production (rCO2) was 1271 (se 4.0) litres/d and the mean energy expenditure was 25 (se 0.8) MJ/d. There were no significant differences in the isotope distribution spaces and flux rates, rH2O, rCO2 or energy expenditure using the multi-point or two-point approaches to calculation. The DLW-derived energy expenditure of 25 MJ/d is approximately 20% higher than the recommended intake of 21 MJ/d for adult hinds kept outdoors (Adam, 1986) and, at 757 kJ/kg0.75 per d, one third higher than the value of 570 kJ/kg0.75 per d for stags penned indoors (Key et al. 1984). Copyright © The Nutrition Society 1998 Energy expenditure nationallicence Doubly-labelled water nationallicence Red deer nationallicence Haggarty P. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK Robinson J. J. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK Ashton J. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK Milne E. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK Adam C. L. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK Kyle C. E. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK Christie S. L. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK Midwood A. J. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK British Journal of Nutrition Cambridge University Press 80/3(1998-09), 263-272 0007-1145 80:3<263 1998 80 BJN https://doi.org/10.1017/S0007114598001317 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0007114598001317 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Haggarty P. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK NATIONALLICENCE 700 1- Robinson J. J. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK NATIONALLICENCE 700 1- Ashton J. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK NATIONALLICENCE 700 1- Milne E. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK NATIONALLICENCE 700 1- Adam C. L. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK NATIONALLICENCE 700 1- Kyle C. E. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK NATIONALLICENCE 700 1- Christie S. L. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK NATIONALLICENCE 700 1- Midwood A. J. Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 80/3(1998-09), 263-272 0007-1145 80:3<263 1998 80 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge