caa a22 4500 388063254 CHVBK 20180307125127.0 cr unu---uuuuu 161130e199807 xx s 000 0 eng 10.1017/S0007114598001834 doi S0007114598001834 pii (NATIONALLICENCE)cambridge-10.1017/S0007114598001834 Actions of the traditional anti-diabetic plant, Agrimony eupatoria (agrimony): effects on hyperglycaemia, cellular glucose metabolism and insulin secretion [Elektronische Daten] Agrimony eupatoria (agrimony) has been documented as a traditional treatment of diabetes. Here, the effects of dietary administration of agrimony on streptozotocin (STZ)-diabetic mice and on in vitro glucose uptake and glucose metabolism, and on insulin secretion by BRIN-BD11 cells were investigated. Agrimony incorporated into the diet (62·5 g/kg) and drinking water (2·5 g/l) countered the weight loss, polydipsia, hyperphagia and hyperglycaemia of STZ-diabetic mice. Aqueous extract of agrimony (1 mg/ml) stimulated 2-deoxy-glucose transport (1·4-fold), glucose oxidation (1·4-fold) and incorporation of glucose into glycogen (2·0-fold) in mouse abdominal muscle comparable with 0·1 μM-insulin. In acute 20 min tests, 0·25-1 mg/ml aqueous extract of agrimony evoked a stepwise 1·9-3·8-fold stimulation of insulin secretion from the BRIN-BD11 pancreatic B-cell line. This effect was abolished by 0·5mM-diazoxide and previous exposure to extract did not adversely affect subsequent stimulation of insulin secretion by 10 mM-L-alanine, thereby indicating that there was no detrimental effect of the extract on cell viability. The effect of extract was glucose-independent and was not evident in BRIN-BD11 cells exposed to a depolarizing concentration of KCl. The ability of agrimony extract to enhance insulin secretion was dependent on use of heat during extract preparation. These results demonstrate the presence of antihyperglycaemic, insulin-releasing and insulin-like activity in Agrimony eupatoria. Copyright © The Nutrition Society 1998 Agrimony nationallicence Insulin nationallicence Diabetes nationallicence Traditional plant therapies nationallicence Gray Alison M. School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK Flatt Peter R. School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK British Journal of Nutrition Cambridge University Press 80/1(1998-07), 109-114 0007-1145 80:1<109 1998 80 BJN https://doi.org/10.1017/S0007114598001834 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0007114598001834 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gray Alison M. School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK NATIONALLICENCE 700 1- Flatt Peter R. School of Biomedical Sciences, University of Ulster, Coleraine BT52 1SA, UK NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 80/1(1998-07), 109-114 0007-1145 80:1<109 1998 80 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge