caa a22 4500 388083875 CHVBK 20180307125225.0 cr unu---uuuuu 161130e199906 xx s 000 0 eng 10.1017/S0022215100144408 doi S0022215100144408 pii (NATIONALLICENCE)cambridge-10.1017/S0022215100144408 Ahluwalia Karvita B. Cell Biology and Electron Microscope Section, Department of Biophysics, All India Institute of Medical Sciences, New Delhi - 110 029, India. Culture of the organism that causes rhinosporidiosis [Elektronische Daten] [Karvita B. Ahluwalia] The causative agent of rhinosporidiosis is a microscopic round body in polypoidal masses that is believed to be the sporangium of a fungus Rhinosporidium seeberi. But fungal aetiology can not be proved with certainty. Attempts to culture the fungus on various media have also been unsuccessful. In a recent study pond water samples, from water in which patients had been bathing, were analysed and a unicellular prokaryotic cyanobacterium Microcystis aeruginosa was isolated. The same cyanobacterium was demonstrated in clinical samples from patients with rhinosporidiosis. Consequent to these findings, attempts were made to culture the causative organism of rhinosporidiosis under conditions that support growth of cyanobacteria. This study describes a simple method for laboratory culture of this organism. Observations based on laser-scanning confocal microscopy, light and electron microscopy confirm that a cyanobacterium, Microcystis sp. is the causative agent of the disease. Rhinosporidiosis is the first human disease to be shown to be caused by a cyanobacterium. The findings have opened the way for development of therapy. Copyright © JLO (1984) Limited 1999 Rhinosporidiosis nationallicence Cyanobacteria nationallicence Culture nationallicence The Journal of Laryngology & Otology Cambridge University Press 113/6(1999-06), 523-528 0022-2151 113:6<523 1999 113 JLO https://doi.org/10.1017/S0022215100144408 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0022215100144408 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Ahluwalia Karvita B. Cell Biology and Electron Microscope Section, Department of Biophysics, All India Institute of Medical Sciences, New Delhi - 110 029, India NATIONALLICENCE 773 0- The Journal of Laryngology & Otology Cambridge University Press 113/6(1999-06), 523-528 0022-2151 113:6<523 1999 113 JLO CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge