caa a22 4500 38809298X CHVBK 20180307125256.0 cr unu---uuuuu 161130e199902 xx s 000 0 eng 10.4039/Ent13193-1 doi S0008347X00009718 pii (NATIONALLICENCE)cambridge-10.4039/Ent13193-1 YIELD OF OCCLUSION BODIES FROM FOREST TENT CATERPILLAR (LEPIDOPTERA: LASIOCAMPIDAE) LARVAE INFECTED WITH A NUCLEAR POLYHEDROSIS VIRUS [Elektronische Daten] The forest tent caterpillar, Malacosoma disstria Hübner (Lepidoptera: Lasiocampidae), is subject to a variety of naturally occurring infectious diseases, including a nuclear polyhedrosis virus (MdNPV) which has been observed to reach epizootic proportions (Stairs 1966; Myers 1993). Because MdNPV is currently available only in limited quantities for small-scale experimentation, production scale-up is vital to it's potential as a biological control agent against the forest tent caterpillar. Viruses replicate only in living cells, therefore it is necessary to produce viral insecticides either in host insect larvae (in vivo) or in susceptible cell cultures (in vitro). Insect cell cultures have not yet been developed so that large-scale production of baculoviruses are feasible (Cunningham 1995), thus in vivo production must be used. To plan for the scale-up of NPV production, it is necessary to establish the yield of occlusion bodies (OBs) from M. disstria larvae. We report here the yield of OBs from four larval instars inoculated with MdNPV, the time to mortality, head capsule widths, and body weights, and recommend larval instars suitable for further research toward commercial-scale production. Copyright © Entomological Society of Canada 1999 Ebling P.M. Canadian Forest Service, Natural Resources Canada, P.O. Box 490, Sault Ste. Marie. Ontario, Canada P6A 5M7 Kaupp W.J. Canadian Forest Service, Natural Resources Canada, P.O. Box 490, Sault Ste. Marie. Ontario, Canada P6A 5M7 The Canadian Entomologist Cambridge University Press 131/1(1999-02), 93-95 0008-347X 131:1<93 1999 131 TCE https://doi.org/10.4039/Ent13193-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.4039/Ent13193-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ebling P.M. Canadian Forest Service, Natural Resources Canada, P.O. Box 490, Sault Ste. Marie. Ontario, Canada P6A 5M7 NATIONALLICENCE 700 1- Kaupp W.J. Canadian Forest Service, Natural Resources Canada, P.O. Box 490, Sault Ste. Marie. Ontario, Canada P6A 5M7 NATIONALLICENCE 773 0- The Canadian Entomologist Cambridge University Press 131/1(1999-02), 93-95 0008-347X 131:1<93 1999 131 TCE CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence SWISSBIB 387959599 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge