caa a22 4500 388093102 CHVBK 20180307125257.0 cr unu---uuuuu 161130e199912 xx s 000 0 eng 10.4039/Ent131715-6 doi S0008347X00011123 pii (NATIONALLICENCE)cambridge-10.4039/Ent131715-6 EVALUATION OF ICE-NUCLEATING MICROORGANISMS FOR REDUCING THE SUPERCOOLING CAPACITY AND COLD-HARDINESS OF CACOPSYLLA PYRICOLA (HEMIPTERA: PSYLLIDAE) [Elektronische Daten] In laboratory studies, suspensions of killed and live ice-nucleating microorganisms were used to decrease the supercooling capacity of the winter form of pear psylla, Cacopsylla pyricola (Foerster) (Hemiptera: Psyllidae). Dry, untreated adults supercooled extensively before they froze at −22.7 °C. Application of 1000 ppm of a preparation of the killed ice-nucleating bacterium, Pseudomonas syringae Van Hall 1902 (Pseudomonadaceae), significantly decreased the adults' supercooling capacity causing some individuals to freeze at temperatures as high as −3.9 °C. Topical application of several live microorganisms also reduced the supercooling capacity of adults significantly; Pseudomonas putida (Trevisan 1989) was the most effective, causing more than 80% of C. pyricola adults to freeze at −15 °C or higher. Furthermore, the temperature of crystallization of adults treated with P. putida remained significantly higher than controls for at least lid post-treatment. Application of ice-nucleating microorganisms also reduced the capacity of adults to survive short-term exposure to high subzero temperatures comparable to a mild frost. Realization of this approach for biological control of pear psylla will require the development of methods for the delivery of microorganisms to overwintering adults under field conditions. Copyright © Entomological Society of Canada 1999 Lee Richard E. Department of Zoology, Miami University, Oxford, Ohio, USA 45056 Litzgus Jacqueline D. Department of Zoology, Miami University, Oxford, Ohio, USA 45056 Mugnano John A. Department of Zoology, Miami University, Oxford, Ohio, USA 45056 Lee Marcia R. Department of Microbiology, Miami University, Oxford, Ohio, USA 45056 Horton David R. USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Rd., Wapato, Washington, USA 98951 Dunley John Tree Fruit Research Center, Washington State University, 1100 North Western Ave., Wenatchee, Washington, USA 98801 The Canadian Entomologist Cambridge University Press 131/6(1999-12), 715-723 0008-347X 131:6<715 1999 131 TCE https://doi.org/10.4039/Ent131715-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.4039/Ent131715-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Richard E. Department of Zoology, Miami University, Oxford, Ohio, USA 45056 NATIONALLICENCE 700 1- Litzgus Jacqueline D. Department of Zoology, Miami University, Oxford, Ohio, USA 45056 NATIONALLICENCE 700 1- Mugnano John A. Department of Zoology, Miami University, Oxford, Ohio, USA 45056 NATIONALLICENCE 700 1- Lee Marcia R. Department of Microbiology, Miami University, Oxford, Ohio, USA 45056 NATIONALLICENCE 700 1- Horton David R. USDA-ARS, Yakima Agricultural Research Laboratory, 5230 Konnowac Pass Rd., Wapato, Washington, USA 98951 NATIONALLICENCE 700 1- Dunley John Tree Fruit Research Center, Washington State University, 1100 North Western Ave., Wenatchee, Washington, USA 98801 NATIONALLICENCE 773 0- The Canadian Entomologist Cambridge University Press 131/6(1999-12), 715-723 0008-347X 131:6<715 1999 131 TCE CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge