caa a22 4500 388093390 CHVBK 20180307125257.0 cr unu---uuuuu 161130e199904 xx s 000 0 eng 10.4039/Ent131229-2 doi S0008347X00009846 pii (NATIONALLICENCE)cambridge-10.4039/Ent131229-2 DNA FINGERPRINTING OF SINGLE APHID EMBRYOS BY RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD-PCR) [Elektronische Daten] Extraction of DNA from whole aphids, in combination with random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) (Williams et al. 1990) markers can detect interspecific and intraspecific genetic variation (Black et al. 1992; Cenis et al. 1993). However, these techniques entail destructive sampling of fresh or preserved specimens. To allow experimental replication from a single sample while preserving the same aphid for morphometrical or karyotyping analyses, we describe a technique for RAPD-PCR using DNA from single aphid embryos. We evaluated the usefulness and reliability of single-embryo analysis, using four species of our laboratory colonies, namely Acyrthosiphon pisum (Harris), Aphis fabae Scopoli, Aphis frangulae group, and Aphis gossypii Glover. Copyright © Entomological Society of Canada 1999 Chan C.K. Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6 Petersen D.J. Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada VOH 1ZO Vrain T.C. Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada VOH 1ZO The Canadian Entomologist Cambridge University Press 131/2(1999-04), 229-230 0008-347X 131:2<229 1999 131 TCE https://doi.org/10.4039/Ent131229-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.4039/Ent131229-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chan C.K. Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6 NATIONALLICENCE 700 1- Petersen D.J. Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada VOH 1ZO NATIONALLICENCE 700 1- Vrain T.C. Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada VOH 1ZO NATIONALLICENCE 773 0- The Canadian Entomologist Cambridge University Press 131/2(1999-04), 229-230 0008-347X 131:2<229 1999 131 TCE CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge