caa a22 4500 388097426 CHVBK 20180307125307.0 cr unu---uuuuu 161130e199911 xx s 000 0 eng 10.1017/S0029665199001287 doi S0029665199001287 pii (NATIONALLICENCE)cambridge-10.1017/S0029665199001287 Landau Bernard R. Departments of Medicine, Biochemistry, and Nutrition, Case Western Reserve University School of Medicine, Cleveland, OH 44106-4951, USA Glycerol production and utilization measured using stable isotopes [Elektronische Daten] [Bernard R. Landau] The rate of appearance of glycerol in the systemic circulation is determined from the enrichment of arterial blood glycerol when labelled glycerol is infused intravenously. This value provides a good measure of whole-body lipolysis during fasting, except that arterial infusion and venous sampling, if feasible, would probably give a higher more-accurate value. Lipolysis occurs primarily in adipose tissue, although other tissues contribute, notably muscle. Measurement is based on the difference in the enrichment of the glycerol entering and leaving the tissue. Lipolysis is underestimated by the extent to which glycerol released by lipolysis does not enter the systemic circulation, as occurs when lipolysis takes place in the non-hepatic tissue of the splanchnic bed. Glycerol released into the systemic circulation is utilized mainly by liver, although kidney and muscle are also major users of glycerol. Measurement of glycerol utilization is based on the amount of labelled glycerol taken up by the tissues. Other tissues probably utilize glycerol to a smaller extent, but in total this represents a significant amount. Most glycerol taken up by liver is converted to glucose. Glucose is probably the major source of glycerol-3-phosphate used in the esterification of fatty acids by adipose tissue. The Nutrition Society Glycerol nationallicence Lipolysis nationallicence Stable isotopes nationallicence Proceedings of the Nutrition Society Cambridge University Press 58/4(1999-11), 973-978 0029-6651 58:4<973 1999 58 PNS https://doi.org/10.1017/S0029665199001287 text/html Onlinezugriff via DOI 1 conference jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0029665199001287 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Landau Bernard R. Departments of Medicine, Biochemistry, and Nutrition, Case Western Reserve University School of Medicine, Cleveland, OH 44106-4951, USA NATIONALLICENCE 773 0- Proceedings of the Nutrition Society Cambridge University Press 58/4(1999-11), 973-978 0029-6651 58:4<973 1999 58 PNS CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge