caa a22 4500 388097434 CHVBK 20180307125307.0 cr unu---uuuuu 161130e199911 xx s 000 0 eng 10.1017/S0029665199001317 doi S0029665199001317 pii (NATIONALLICENCE)cambridge-10.1017/S0029665199001317 Jackson Malcolm J. Department of Medicine, University of Liverpool, Liverpool L69 3GA, UK An overview of methods for assessment of free radical activity in biology [Elektronische Daten] [Malcolm J. Jackson] Assays which purport to assess free radical activity in biological systems are multiple. However, despite numerous published descriptions of new methods and modifications of methods to assess free radical activity in biological materials, there is still a lack of reliable techniques for quantification of activity in vivo. Analysis of a number of related indicators and use of a variety of approaches appears the only reliable way to evaluate these processes in vivo. In studies of free radical generation by contracting skeletal muscle we have attempted to use a variety of indicators, including measurement of endogenous antioxidant levels, measurement of indirect indicators of free radical activity (e.g. products of lipid peroxidation, DNA oxidation or protein oxidation) and, where possible, measurement of direct indicators of free radical activity by electron spin resonance techniques. In view of the relative lack of specificity of many available techniques, caution should be exerted in evaluating the numerous examples of isolated single measures of free radical activity which are present in the scientific literature. The Nutrition Society Free radicals nationallicence Electron spin resonance techniques nationallicence Oxidative stress nationallicence Antioxidants nationallicence Proceedings of the Nutrition Society Cambridge University Press 58/4(1999-11), 1001-1006 0029-6651 58:4<1001 1999 58 PNS https://doi.org/10.1017/S0029665199001317 text/html Onlinezugriff via DOI 1 conference jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0029665199001317 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Jackson Malcolm J. Department of Medicine, University of Liverpool, Liverpool L69 3GA, UK NATIONALLICENCE 773 0- Proceedings of the Nutrition Society Cambridge University Press 58/4(1999-11), 1001-1006 0029-6651 58:4<1001 1999 58 PNS CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge