caa a22 4500 388097507 CHVBK 20180307125307.0 cr unu---uuuuu 161130e199911 xx s 000 0 eng 10.1017/S0029665199001330 doi S0029665199001330 pii (NATIONALLICENCE)cambridge-10.1017/S0029665199001330 Duthie Garry G. Rowett Research Institute, Greenburn Road, Aberdeen AB21 9SB, UK Determination of activity of antioxidants in human subjects [Elektronische Daten] [Garry G. Duthie] Evidence from biochemical and animal models suggests that nutritional antioxidants should inhibit the development of diseases such as CHD and certain cancers. This evidence is not clearly corroborated by intervention studies in human subjects, due, in part, to inadequacies in current analytical methodologies. Although in vitro assays can give useful information on the attributes required by a compound to act as an antioxidant, results may have little nutritional relevance due to limited bioavailability. The determination of antioxidants in blood is often used as a measure of antioxidant status in vivo, but may not necessarily reflect concentrations in target tissues where oxidative stress is greatest. In addition, the accumulation of antioxidants in selective tissues may not be apparent from plasma measurements. Participation in quality-control schemes for antioxidant determination by HPLC allows inter-laboratory comparison of results. Moderation of indices of oxidative damage to lipids, proteins and DNA can provide information on the effectiveness of compounds as nutritional antioxidants. However, most current methods of assessing oxidative stress are subject to confounding factors of non-oxidative origin. Assays for total antioxidant capacity in plasma differ in their type of oxidation source, target and measurement used to detect the oxidized product. They give different results, should never be used in isolation, and results should be interpreted with caution. Until more is known about the activity and metabolic fate of antioxidants, caution should be exercised in the consumption of large amounts of commercially-available antioxidant preparations. The Nutrition Society Antioxidants nationallicence Oxidative damage nationallicence Proceedings of the Nutrition Society Cambridge University Press 58/4(1999-11), 1015-1024 0029-6651 58:4<1015 1999 58 PNS https://doi.org/10.1017/S0029665199001330 text/html Onlinezugriff via DOI 1 conference jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0029665199001330 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Duthie Garry G. Rowett Research Institute, Greenburn Road, Aberdeen AB21 9SB, UK NATIONALLICENCE 773 0- Proceedings of the Nutrition Society Cambridge University Press 58/4(1999-11), 1015-1024 0029-6651 58:4<1015 1999 58 PNS CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge