caa a22 4500 388097701 CHVBK 20180307125308.0 cr unu---uuuuu 161130e199911 xx s 000 0 eng 10.1017/S0029665199001251 doi S0029665199001251 pii (NATIONALLICENCE)cambridge-10.1017/S0029665199001251 Westerterp Klaas R. Department of Human Biology, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands Body composition, water turnover and energy turnover assessment with labelled water [Elektronische Daten] [Klaas R. Westerterp] Our understanding of human energy metabolism has benefited greatly from the application of water labelled with 2H and 18O for the measurement of total body water, water turnover and total daily energy expenditure. Applications include validation of techniques for the assessment of dietary intake and physical activity, assessment of water and energy requirement and the assessment of the effect of dietary and physical activity interventions, including its use with endurance athletes competing at the highest level. Critical aspects of the application are isotope dose preparation, sample collection, sample analysis and the calculation procedure. The labelled-water method can easily be applied in normal living conditions, including exercise, and in the clinical setting. However, sample analysis requires a sophisticated laboratory with an isotope-ratio mass spectrometer and a sample preparation system. Examples of insights based on labelled-water studies are: (1) self-reported dietary intakes often underestimate energy requirements; (2) subjects have problems maintaining energy balance when daily energy expenditure exceeds 2.5 times resting energy expenditure. Devices for the assessment of physical activity validated using labelled water allow the study of activity patterns and strategies to influence the activity level of a sedentary society. The Nutrition Society Energy intake nationallicence Energy requirement nationallicence Energy balance nationallicence Exercise nationallicence Doubly-labelled water nationallicence Proceedings of the Nutrition Society Cambridge University Press 58/4(1999-11), 945-951 0029-6651 58:4<945 1999 58 PNS https://doi.org/10.1017/S0029665199001251 text/html Onlinezugriff via DOI 1 conference jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0029665199001251 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Westerterp Klaas R. Department of Human Biology, Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands NATIONALLICENCE 773 0- Proceedings of the Nutrition Society Cambridge University Press 58/4(1999-11), 945-951 0029-6651 58:4<945 1999 58 PNS CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge