caa a22 4500 388112220 CHVBK 20180307125356.0 cr unu---uuuuu 161130e199902 xx s 000 0 eng 10.1017/S0022149X99000244 doi S0022149X99000244 pii (NATIONALLICENCE)cambridge-10.1017/S0022149X99000244 Studies on the viability of metacercariae of Fasciola gigantica [Elektronische Daten] The viability of metacercariae of Fasciola gigantica was tested by in vitro and in vivo methods. In vitro testing was based upon the motility of juvenile flukes within the inner cyst as examined under the light microscope. In vivo testing was undertaken through experimental infections of rabbits (two groups) and natural definitive hosts, lambs (one group). In the first group, out of six rabbits each given 25 metacercariae, worm establishment only took place in one rabbit with a single fluke recovery on 60 days post infection. In the second group of six rabbits each given 200 metacercariae, five were infected, with two or three flukes per host. All the lambs given 250 metacercariae became infected showing prevalences of 7.2-40% in comparison with rabbits in which low prevalences (0-4%) were recorded. The results indicated that even viable metacercariae which were already tested in vitro could not readily establish in rabbits. Such variability in worm establishment suggests that immunological and chemotherapeutic studies in rabbits infected with F. gigantica are likely to be unreliable. Cambridge University Press Prasad A. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India Ghosh S. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India Bhatnagar P.K. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India Santra P.K. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India Journal of Helminthology Cambridge University Press 73/2(1999-02), 163-166 0022-149X 73:2<163 1999 73 JHL https://doi.org/10.1017/S0022149X99000244 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0022149X99000244 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Prasad A. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India NATIONALLICENCE 700 1- Ghosh S. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India NATIONALLICENCE 700 1- Bhatnagar P.K. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India NATIONALLICENCE 700 1- Santra P.K. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243 122, India NATIONALLICENCE 773 0- Journal of Helminthology Cambridge University Press 73/2(1999-02), 163-166 0022-149X 73:2<163 1999 73 JHL CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge