caa a22 4500 388112247 CHVBK 20180307125356.0 cr unu---uuuuu 161130e199902 xx s 000 0 eng 10.1017/S0022149X99000189 doi S0022149X99000189 pii (NATIONALLICENCE)cambridge-10.1017/S0022149X99000189 Proteolytic activity in Caenorhabditis elegans [Elektronische Daten] soluble and insoluble fractions Proteolytic activities of soluble and insoluble fractions of the free-living soil nematode Caenorhabditis elegans were measured across a range of substrates, temperatures and pH conditions. Several protease inhibitors were also tested under these conditions. Results of these studies indicate that proteolytic activity is present in cytosolic (CF) and non-cytosolic (NCF) fractions of C. elegans extracts at every condition of pH, temperature and buffer assayed. On the other hand, the use of different protease inhibitors demonstrated the existence of exo and endoproteases types in CF as well as NCF. Moreover our results show that the use of two protease inhibitor mixed types proposed by several authors are not enough to avoid this lytic activity when homogenates of this nematode are employed in biochemical assays. Cambridge University Press Gimenez-Pardo C. Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Crtra. Madrid-Barcelona km 33, 28871 Alcala de Henares, Madrid, Spain Vazquez-Lopez C. Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Crtra. Madrid-Barcelona km 33, 28871 Alcala de Henares, Madrid, Spain de Armas-Serra C. Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Crtra. Madrid-Barcelona km 33, 28871 Alcala de Henares, Madrid, Spain Rodriguez-Caabeiro F. Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Crtra. Madrid-Barcelona km 33, 28871 Alcala de Henares, Madrid, Spain Journal of Helminthology Cambridge University Press 73/2(1999-02), 123-127 0022-149X 73:2<123 1999 73 JHL https://doi.org/10.1017/S0022149X99000189 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0022149X99000189 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gimenez-Pardo C. Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Crtra. Madrid-Barcelona km 33, 28871 Alcala de Henares, Madrid, Spain NATIONALLICENCE 700 1- Vazquez-Lopez C. Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Crtra. Madrid-Barcelona km 33, 28871 Alcala de Henares, Madrid, Spain NATIONALLICENCE 700 1- de Armas-Serra C. Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Crtra. Madrid-Barcelona km 33, 28871 Alcala de Henares, Madrid, Spain NATIONALLICENCE 700 1- Rodriguez-Caabeiro F. Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Crtra. Madrid-Barcelona km 33, 28871 Alcala de Henares, Madrid, Spain NATIONALLICENCE 773 0- Journal of Helminthology Cambridge University Press 73/2(1999-02), 123-127 0022-149X 73:2<123 1999 73 JHL CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge