caa a22 4500 388112344 CHVBK 20180307125356.0 cr unu---uuuuu 161130e199903 xx s 000 0 eng 10.1017/S0022149X99000384 doi S0022149X99000384 pii (NATIONALLICENCE)cambridge-10.1017/S0022149X99000384 Hepatic [2-13C]acetate metabolism by jirds infected with Echinococcus multilocularis [Elektronische Daten] Carbon-13 decoupled 1H spin echo NMR spectroscopy, with and without population inversion, was used to study carbon flow between the host, Meriones unguiculatus, and the parasite, Echinococcus multilocularis. This was accomplished by monitoring [2-13C]acetate metabolism in the liver of jirds infected with metacestodes of this parasite. Thirty minutes after injection of labelled acetate solution into the portal vein, 13C enrichment was observed in hepatic acetate, β-hydroxybutyrate, succinate, alanine, lactate and glucose. For E. multilocularis cysts, at this time,13C enrichment was observed in the same metabolites as in livers and, in addition, citrate. At 120 min there was a significant decrease in the amount of label present in all hepatic metabolites whereas more label was found in the majority of the parasite metabolites. The results confirm that exogenous acetate, through randomization of the 13C in biochemical pathways of host liver, ends up in hepatic glucose. As this biosynthetic route is not available to the parasite, the presence of 13C enriched glucose in the cysts clearly indicates that the parasite is siphoning off glucose that is newly synthesized by the host. At 120 min some of this labelled glucose was stored in parasite glycogen whereas some of it had been catabolized to succinate, alanine, lactate and acetate, end products which are excreted back into the host. Cambridge University Press Schoen J. Department of Biology, University of Winnipeg, Winnipeg, Manitoba, Canada R3B 2E9 Novak M. Department of Biology, University of Winnipeg, Winnipeg, Manitoba, Canada R3B 2E9 Blackburn B.J. Department of Chemistry, University of Winnipeg, Winnipeg, Manitoba, Canada R3B 2E9 Journal of Helminthology Cambridge University Press 73/3(1999-03), 245-250 0022-149X 73:3<245 1999 73 JHL https://doi.org/10.1017/S0022149X99000384 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0022149X99000384 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schoen J. Department of Biology, University of Winnipeg, Winnipeg, Manitoba, Canada R3B 2E9 NATIONALLICENCE 700 1- Novak M. Department of Biology, University of Winnipeg, Winnipeg, Manitoba, Canada R3B 2E9 NATIONALLICENCE 700 1- Blackburn B.J. Department of Chemistry, University of Winnipeg, Winnipeg, Manitoba, Canada R3B 2E9 NATIONALLICENCE 773 0- Journal of Helminthology Cambridge University Press 73/3(1999-03), 245-250 0022-149X 73:3<245 1999 73 JHL CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge