caa a22 4500 388112409 CHVBK 20180307125357.0 cr unu---uuuuu 161130e199903 xx s 000 0 eng 10.1017/S0022149X99000426 doi S0022149X99000426 pii (NATIONALLICENCE)cambridge-10.1017/S0022149X99000426 Polymorphism in the Dirofilaria immitis immunodominant antigen gene [Elektronische Daten] Dg2, a gene encoding a 34 kDa immunodominant antigen of Dirofilaria immitis was cloned and demonstrated to be specifically expressed in the larval stage. In this study, a newly constructed genomic DNA library was screened by hybridization with Dg2. One of the resulting positive clones was similar to Dg2 in the structure of its exonic regions but different in number, position, size and sequence of introns. This was designated DgK. Full-length cDNA was isolated using the rapid amplification of cDNA ends (RACE) method to study the transcript corresponding to DgK. Sequence analysis revealed that the mRNA corresponding to DgK is trans-spliced during post-transcriptional processing because the 5′ end of the amplified cDNA contains seven nucleotides of the nematode-spliced leader (SL) sequence. Cambridge University Press Sugane K. Department of Parasitology Nakayama K. Department of Anatomy, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto City, Nagano Prefecture 390, Japan Kato H. Department of Bioscience Fukui Prefecture, University School of Bioscience, Fukui, Japan Journal of Helminthology Cambridge University Press 73/3(1999-03), 265-272 0022-149X 73:3<265 1999 73 JHL https://doi.org/10.1017/S0022149X99000426 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0022149X99000426 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sugane K. Department of Parasitology NATIONALLICENCE 700 1- Nakayama K. Department of Anatomy, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto City, Nagano Prefecture 390, Japan NATIONALLICENCE 700 1- Kato H. Department of Bioscience Fukui Prefecture, University School of Bioscience, Fukui, Japan NATIONALLICENCE 773 0- Journal of Helminthology Cambridge University Press 73/3(1999-03), 265-272 0022-149X 73:3<265 1999 73 JHL CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge