Quantitation of Simian Cytokine andβ-Chemokine mRNAs, using real-time reverse transcriptase-polymerase chain reaction: variations in expression during chronic primate lentivirus infection
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| 005 | 20181103044323.0 | ||
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| 008 | 161201s2002 xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.5167/uzh-128334 |2 doi |
| 024 | 7 | 0 | |a 10.1089/088922202760019329 |2 doi |
| 035 | |a (ZORA)oai:www.zora.uzh.ch:128334 | ||
| 084 | |a 570 |2 ddc | ||
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| 245 | 0 | 0 | |a Quantitation of Simian Cytokine andβ-Chemokine mRNAs, using real-time reverse transcriptase-polymerase chain reaction: variations in expression during chronic primate lentivirus infection |h [Elektronische Daten] |c [Regina Hofmann-Lehmann, Alison L Williams, Ryan K Swenerton, Pei-Lin Li, Robert A Rasmussen, Agnès-Laurence Chenine, Harold M McClure, Ruth M Ruprecht] |
| 506 | |a restrictedAccess |2 eu-repo | ||
| 520 | 3 | |a Cytokines and β-chemokines are important mediators of the immune system and are expressed in many infectious diseases. To study cytokine and β-chemokine profiles during pathogenesis of lentiviral infection and progression to AIDS in rhesus macaques, we established new quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assays based on TaqMan chemistry. Using synthetic RNA standards, we quantified mRNAs of IL-2, IL-4, IL-6, IL-10, IL-12 p40, interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), RANTES, macrophage inflammatory protein 1α (MIP-1α), and MIP-1β in unstimulated peripheral blood mononuclear cells (PBMCs) and lymph nodes from macaques chronically infected with SIV or SHIV. Viremic monkeys with decreased CD4+ T cell counts (<500 cells/μl) had significantly higher IL-10 mRNA expression than uninfected controls, which parallels the findings in HIV-1-infected humans. In addition, MIP-1α, MIP-1β, and RANTES mRNA expression increased in viremic monkeys with decreased CD4+ T cell counts; gene expression was inversely correlated with CD4+ T cell counts, but not viral load. The newly established quantitative real-time RT-PCR assays will allow the determination of cytokine and β-chemokine patterns in rhesus macaques in studies of microbial pathogenesis or vaccine development. | |
| 690 | 7 | |a Department of Farm Animals |2 zora | |
| 690 | 7 | |a Immunology, Virology, Infectious Diseases |2 zora | |
| 700 | 1 | |a Hofmann-Lehmann |D Regina |e joint author | |
| 700 | 1 | |a Williams |D Alison L. |e joint author | |
| 700 | 1 | |a Swenerton |D Ryan K. |e joint author | |
| 700 | 1 | |a Li |D Pei-Lin |e joint author | |
| 700 | 1 | |a Rasmussen |D Robert A. |e joint author | |
| 700 | 1 | |a Chenine |D Agnès-Laurence |e joint author | |
| 700 | 1 | |a McClure |D Harold M. |e joint author | |
| 700 | 1 | |a Ruprecht |D Ruth M. |e joint author | |
| 773 | 0 | |t AIDS Research and Human Retroviruses |g 18(9):627-639 | |
| 856 | 4 | 0 | |u https://www.zora.uzh.ch/id/eprint/128334/1/AIDS%20Research.pdf |q text/html |z WWW-Backlink auf das Repository |
| 908 | |D 1 |a Journal Article |z PeerReviewed |2 zora | ||
| 950 | |B ZORA |P 856 |E 40 |u https://www.zora.uzh.ch/id/eprint/128334/1/AIDS%20Research.pdf |q text/html |z WWW-Backlink auf das Repository | ||
| 950 | |B ZORA |P 700 |E 1- |a Hofmann-Lehmann |D Regina |e joint author | ||
| 950 | |B ZORA |P 700 |E 1- |a Williams |D Alison L. |e joint author | ||
| 950 | |B ZORA |P 700 |E 1- |a Swenerton |D Ryan K. |e joint author | ||
| 950 | |B ZORA |P 700 |E 1- |a Li |D Pei-Lin |e joint author | ||
| 950 | |B ZORA |P 700 |E 1- |a Rasmussen |D Robert A. |e joint author | ||
| 950 | |B ZORA |P 700 |E 1- |a Chenine |D Agnès-Laurence |e joint author | ||
| 950 | |B ZORA |P 700 |E 1- |a McClure |D Harold M. |e joint author | ||
| 950 | |B ZORA |P 700 |E 1- |a Ruprecht |D Ruth M. |e joint author | ||
| 950 | |B ZORA |P 773 |E 0- |t AIDS Research and Human Retroviruses |g 18(9):627-639 | ||
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 949 | |B ZORA |F ZORA |b ZORA |j Journal Article |c restrictedAccess | ||