cad a22 4500 390148032 20181103044323.0 cr unu---uuuuu 161201s2002 xx s 000 0 eng 10.5167/uzh-128334 doi 10.1089/088922202760019329 doi (ZORA)oai:www.zora.uzh.ch:128334 570 ddc 630 ddc 12079558 pmid Quantitation of Simian Cytokine andβ-Chemokine mRNAs, using real-time reverse transcriptase-polymerase chain reaction: variations in expression during chronic primate lentivirus infection [Elektronische Daten] [Regina Hofmann-Lehmann, Alison L Williams, Ryan K Swenerton, Pei-Lin Li, Robert A Rasmussen, Agnès-Laurence Chenine, Harold M McClure, Ruth M Ruprecht] restrictedAccess eu-repo Cytokines and β-chemokines are important mediators of the immune system and are expressed in many infectious diseases. To study cytokine and β-chemokine profiles during pathogenesis of lentiviral infection and progression to AIDS in rhesus macaques, we established new quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assays based on TaqMan chemistry. Using synthetic RNA standards, we quantified mRNAs of IL-2, IL-4, IL-6, IL-10, IL-12 p40, interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), RANTES, macrophage inflammatory protein 1α (MIP-1α), and MIP-1β in unstimulated peripheral blood mononuclear cells (PBMCs) and lymph nodes from macaques chronically infected with SIV or SHIV. Viremic monkeys with decreased CD4+ T cell counts (<500 cells/μl) had significantly higher IL-10 mRNA expression than uninfected controls, which parallels the findings in HIV-1-infected humans. In addition, MIP-1α, MIP-1β, and RANTES mRNA expression increased in viremic monkeys with decreased CD4+ T cell counts; gene expression was inversely correlated with CD4+ T cell counts, but not viral load. The newly established quantitative real-time RT-PCR assays will allow the determination of cytokine and β-chemokine patterns in rhesus macaques in studies of microbial pathogenesis or vaccine development. Department of Farm Animals zora Immunology, Virology, Infectious Diseases zora Hofmann-Lehmann Regina joint author Williams Alison L. joint author Swenerton Ryan K. joint author Li Pei-Lin joint author Rasmussen Robert A. joint author Chenine Agnès-Laurence joint author McClure Harold M. joint author Ruprecht Ruth M. joint author AIDS Research and Human Retroviruses 18(9):627-639 https://www.zora.uzh.ch/id/eprint/128334/1/AIDS%20Research.pdf text/html WWW-Backlink auf das Repository 1 Journal Article PeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/id/eprint/128334/1/AIDS%20Research.pdf text/html WWW-Backlink auf das Repository ZORA 700 1- Hofmann-Lehmann Regina joint author ZORA 700 1- Williams Alison L. joint author ZORA 700 1- Swenerton Ryan K. joint author ZORA 700 1- Li Pei-Lin joint author ZORA 700 1- Rasmussen Robert A. joint author ZORA 700 1- Chenine Agnès-Laurence joint author ZORA 700 1- McClure Harold M. joint author ZORA 700 1- Ruprecht Ruth M. joint author ZORA 773 0- AIDS Research and Human Retroviruses 18(9):627-639 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article restrictedAccess