cad a22 4500 390148059 20181103044323.0 cr unu---uuuuu 161201e20020412xx s 000 0 eng 10.5167/uzh-128336 doi 10.1097/00002030-200204120-00002 doi (ZORA)oai:www.zora.uzh.ch:128336 570 ddc 630 ddc 11919484 pmid Neutralizing antibodies as a potential secondary protective mechanism during chronic SHIV infection in CD8+ T-cell-depleted macaques [Elektronische Daten] [Robert A Rasmussen, Regina Hofmann-Lehmann, Pei-Lin Li, Josef Vlasak, Joern E Schmitz, Keith A Reimann, Marcelo J Kuroda, Norman L Letvin, David C Montefiori, Harold M McClure, Ruth M Ruprecht] closedAccess eu-repo OBJECTIVE: To directly examine the role of CD8+ T cells in controlling viremia and disease during chronic, low-level primate immunodeficiency virus infection in DNA prime/protein boost-vaccinated macaques. BACKGROUND: A cohort of macaques, vaccinated with either a DNA prime/HIV-1 gp160 boost regimen or with gp160 alone was previously protected partially from sequential challenges with non-pathogenic and pathogenic strains of chimeric simian/human immunodeficiency virus (SHIV). In this study, the effect of temporary ablation of CD8+ T cells in these animals was examined. METHODS: Animals were treated with an anti-CD8 antibody and CD8+ T-cell levels in peripheral blood, plasma viral loads, peripheral blood mononuclear cell-associated virus levels, neutralizing antibody (nAb) titers and simian immunodeficiency virus Gag-specific CD8+ T-cell numbers were followed. RESULTS: Plasma viremia rose sharply in direct synchrony with a rapid but transient drop in CD8+ T cells. However, although levels of cell-associated virus also rose concomitantly, peak levels were much lower than those in virus-challenged, naive animals. In addition, despite a rise of pathogenic SHIV89.6P RNA levels in three animals, CD4+ T-cell counts remained unchanged. In each of these animals, neutralizing antibody titers against the pathogenic SHIV89.6P strain were high. CONCLUSIONS: The results indicate that CD8+ T cells play a key role in suppressing viremia in a chronically infected host. In addition, the results suggest that in the absence of CD8+ T cells, nAb may act as an effective second line of defense by limiting both the spread of infectious virus to new target cells and CD4+ T-cell loss. Department of Farm Animals zora Rasmussen Robert A. joint author Hofmann-Lehmann Regina joint author Li Pei-Lin joint author Vlasak Josef joint author Schmitz Joern E. joint author Reimann Keith A. joint author Kuroda Marcelo J. joint author Letvin Norman L. joint author Montefiori David C. joint author McClure Harold M. joint author Ruprecht Ruth M. joint author AIDS 16(6):829-838 https://www.zora.uzh.ch/id/eprint/128336/1/Neutralizing_antibodies_as_a_potential_secondary.2.pdf text/html WWW-Backlink auf das Repository 1 Journal Article PeerReviewed zora ZORA 856 40 https://www.zora.uzh.ch/id/eprint/128336/1/Neutralizing_antibodies_as_a_potential_secondary.2.pdf text/html WWW-Backlink auf das Repository ZORA 700 1- Rasmussen Robert A. joint author ZORA 700 1- Hofmann-Lehmann Regina joint author ZORA 700 1- Li Pei-Lin joint author ZORA 700 1- Vlasak Josef joint author ZORA 700 1- Schmitz Joern E. joint author ZORA 700 1- Reimann Keith A. joint author ZORA 700 1- Kuroda Marcelo J. joint author ZORA 700 1- Letvin Norman L. joint author ZORA 700 1- Montefiori David C. joint author ZORA 700 1- McClure Harold M. joint author ZORA 700 1- Ruprecht Ruth M. joint author ZORA 773 0- AIDS 16(6):829-838 BK010053 XK010053 XK010000 ZORA ZORA ZORA Journal Article closedAccess