caa a22 4500 397493894 CHVBK 20180308164515.0 cr unu---uuuuu 161202e199509 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124952 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124952 Copy-Dependent and Position-Independent Expression of Rat Aldolase A Gene [Elektronische Daten] [Kazuo Hashido, Yuji Arai, Susumu Kajihara, Kei-ichiro Joh, Hitomi Yatsuki, Katsuji Hori, Jun-ichi Miyazaki, Ken-ichi Yamamura, Tsunehiro Mukai] In order to understand the molecular mechanisms of the temporal and spatial differences of gene expression in higher organisms, rat aldolase A gene carrying two distinct promoters was introduced into fertilized eggs and the resulting transgenic mice were analyzed. The transgene expression is tissue-specific and is developmentally regulated. In addition, the expression is regulated in a copy-dependent manner irrespective of where the transgene is integrated, suggesting that a mechanism excluding the effect of the integration site exists within the transgene itself. To explore the conformational change of this gene in the genome, the DNase I hypersensitive sites of the gene were examined. Three sites(DHS-1, 2, and 3) were identified upstream and downstream of the gene and these sites were retained in the transgene as well as in the gene observed endogenously. © by the Journal of Biochemistry Regular Papers nationallicence Key words nationallicence aldolase A gene nationallicence copy-dependent expression nationallicence DNase I hypesensitive site nationallicence transgene nationallicence transgenic mouse nationallicence Hashido Kazuo Department of Bioscience, National Cardiovascular Center Research Institute 5-7-1 Fujishiro-dai, Suita, Osaka565 aut Arai Yuji Department of Bioscience, National Cardiovascular Center Research Institute 5-7-1 Fujishiro-dai, Suita, Osaka565 aut Kajihara Susumu Department of Bioscience, National Cardiovascular Center Research Institute 5-7-1 Fujishiro-dai, Suita, Osaka565 aut Joh Kei-ichiro Department of Biochemistry, Saga Medical School Saga, Saga 849 aut Yatsuki Hitomi Department of Biochemistry, Saga Medical School Saga, Saga 849 aut Hori Katsuji Department of Biochemistry, Saga Medical School Saga, Saga 849 aut Miyazaki Jun-ichi Institute for Medical Genetics, Kumamoto University Medical School 4-24-1 Kuhonji, Kumamoto, Kumamoto 862 aut Yamamura Ken-ichi Institute for Medical Genetics, Kumamoto University Medical School 4-24-1 Kuhonji, Kumamoto, Kumamoto 862 aut Mukai Tsunehiro Department of Bioscience, National Cardiovascular Center Research Institute 5-7-1 Fujishiro-dai, Suita, Osaka565 aut The Journal of Biochemistry Oxford University Press 118/3(1995-09), 601-606 0021-924X 118:3<601 1995 118 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124952 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124952 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hashido Kazuo Department of Bioscience, National Cardiovascular Center Research Institute 5-7-1 Fujishiro-dai, Suita, Osaka565 aut NATIONALLICENCE 700 1- Arai Yuji Department of Bioscience, National Cardiovascular Center Research Institute 5-7-1 Fujishiro-dai, Suita, Osaka565 aut NATIONALLICENCE 700 1- Kajihara Susumu Department of Bioscience, National Cardiovascular Center Research Institute 5-7-1 Fujishiro-dai, Suita, Osaka565 aut NATIONALLICENCE 700 1- Joh Kei-ichiro Department of Biochemistry, Saga Medical School Saga, Saga 849 aut NATIONALLICENCE 700 1- Yatsuki Hitomi Department of Biochemistry, Saga Medical School Saga, Saga 849 aut NATIONALLICENCE 700 1- Hori Katsuji Department of Biochemistry, Saga Medical School Saga, Saga 849 aut NATIONALLICENCE 700 1- Miyazaki Jun-ichi Institute for Medical Genetics, Kumamoto University Medical School 4-24-1 Kuhonji, Kumamoto, Kumamoto 862 aut NATIONALLICENCE 700 1- Yamamura Ken-ichi Institute for Medical Genetics, Kumamoto University Medical School 4-24-1 Kuhonji, Kumamoto, Kumamoto 862 aut NATIONALLICENCE 700 1- Mukai Tsunehiro Department of Bioscience, National Cardiovascular Center Research Institute 5-7-1 Fujishiro-dai, Suita, Osaka565 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 118/3(1995-09), 601-606 0021-924X 118:3<601 1995 118 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford