caa a22 4500 397493916 CHVBK 20180308164516.0 cr unu---uuuuu 161202e199501 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124725 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124725 Expression of Differentiation-Related Phenotypes and Apoptosis Are Independently Regulated during Myeloid Cell Differentiation [Elektronische Daten] [Yasuhito Terui, Yusuke Furukawa, Kumi Sakoe, Masatsugu Ohta, Masaki Saito] When human promyelocytic leukemia cell line HL-60 was treated with various differentiation-inducers, apoptosis always occurred after the full appearance of differentiation-related phenotypes. However, the two phenomena could be dissociated when HL-60 cells were treated with PDBu. When HL-60 cells were cultured with PDBu for more than 36 h, apoptosis was induced following differentiation. Apoptosis was not, however, observed when PDBu was removed within 24 h, even though induction of differentiation-related phenotypes, such as NBT-reducing ability and surface marker expression, was the same as that in the control. Northern blot analysis revealed that bcl-2 mRNA was rapidly down-regulated within 6 h of the treatment with PDBu. The amount of bcl-2 mRNA recovered to that of undifferentiated HL-60 cells when PDBu was washed out within 24 h. In contrast, the recovery of bcl-2 was incomplete when the cells were treated with PDBu for more than 36 h, suggesting that bcl-2 is also a critical regulator of the cell fate during myeloid differentiation. This hypothesis was confirmed by experiments using antisense oligonucleotides, i.e., blocking the recovery of bcl-2 mRNA by antisense oligonucleotides could result in the induction of apoptosis in HL-60 cells from which PDBu was removed within 24 h. Moreover, overexpression of BCL-2 in HL-60 cells could block apoptosis during differentiation without any significant effect on differentiation itself. These results strongly suggest that apoptosis is not a simple consequence of differentiation-induction, and that apoptosis and differentiation are regulated independently in myeloid cells. © 1995 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence apoptosis nationallicence bcl-2 nationallicence differentiation nationallicence HL-60, overexpression nationallicence Terui Yasuhito Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi 329-04 aut Furukawa Yusuke Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi 329-04 aut Sakoe Kumi Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi 329-04 aut Ohta Masatsugu Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi 329-04 aut Saito Masaki Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi 329-04 aut The Journal of Biochemistry Oxford University Press 117/1(1995-01), 77-84 0021-924X 117:1<77 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124725 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124725 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Terui Yasuhito Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi 329-04 aut NATIONALLICENCE 700 1- Furukawa Yusuke Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi 329-04 aut NATIONALLICENCE 700 1- Sakoe Kumi Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi 329-04 aut NATIONALLICENCE 700 1- Ohta Masatsugu Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi 329-04 aut NATIONALLICENCE 700 1- Saito Masaki Division of Hemopoiesis, Institute of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi 329-04 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/1(1995-01), 77-84 0021-924X 117:1<77 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford