caa a22 4500 397493924 CHVBK 20180308164516.0 cr unu---uuuuu 161202e199505 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124829 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124829 Binding of Myosin and Its Subfragment-1 with Antibodies Specific to the Two Heads of the Myosin Molecule [Elektronische Daten] [Shin Murai, Toshiaki Arata, Akio Inoue] It was shown by Miyanishi et al. [Miyanishi, T., Maita, T., Matsuda, G., and Tonomura, Y. (1982) J. Biochem. 91, 1845-1853] that the amino acid sequence around the reactive lysine residue is different between head B (P1-burst head) and head A of the myosin molecule. Thus, we synthesized these two peptides, and prepared rabbit polyclonal antibodies against them. Each antibody bound strongly with both peptides. However, the binding of the antibodies with S-1 was inhibited by the peptide used for the antigen but unaffected by the non-antigen peptide, suggesting that only antibodies specific to each head can bind with S-1. Myosin was absorbed by either antibody A or B, which was immobilized on protein A in Staphylococcus aureus cells. However, half of S-1 was absorbed by each of the antibodies. The S-1 prepared showed about 0.5 mol of initial P1-liberation per mol of S-1. The P1-burst size of S-1 unbound to the immobilized anti-A antibody increased to almost 1 mol/mol S-1, while that of S-1 unbound to the anti-B antibody decreased to 0.15 mol/mol S-1. These results suggest the existence of two kinds of heads in the myosin molecule © Oxford University Press Regular Papers nationallicence antibody nationallicence myosin nationallicence myosin heads nationallicence muscle nationallicence subfragment-1 nationallicence Murai Shin Department of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka 560 aut Arata Toshiaki Department of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka 560 aut Inoue Akio Department of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka 560 aut The Journal of Biochemistry Oxford University Press 117/5(1995-05), 974-979 0021-924X 117:5<974 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124829 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124829 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Murai Shin Department of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka 560 aut NATIONALLICENCE 700 1- Arata Toshiaki Department of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka 560 aut NATIONALLICENCE 700 1- Inoue Akio Department of Biology, Faculty of Science, Osaka University, Toyonaka, Osaka 560 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/5(1995-05), 974-979 0021-924X 117:5<974 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford