caa a22 4500 397493940 CHVBK 20180308164516.0 cr unu---uuuuu 161202e199512 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124998 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124998 Expression of Human Metallothionein-2 in Escherichia coli: Cadmium Tolerance of Transformed Cells [Elektronische Daten] [Fumitomo Odawara, Masaaki Kurasaki, Mika Suzuki-Kurasaki, Shinji Oikawa, Tadasu Emoto, Futoshi Yamasaki, Ana Rosa Linde Arias, Yutaka Kojima] A genetic approach was undertaken to investigate the physiological roles of human metallothionein-2. A constructed expression plasmid, pEXPMTII, in which human metallothionein-IIA cDNA was inserted downstream of a tryptophan-lactose promoter, was used to transform Escherichia coli JM105 strain. Cadmium-binding metallothionein was successfully expressed in E. coli in the medium containing cadmium, while copper and zinc-metallothioneins were scarcely observed in copper- or zinc-containing medium. The amino acid composition and sequence of the biosynthesized cadmium-metallothionein were analyzed. The selectivity of metals bound to metallothionein and the stability of metal-binding forms of metallothionein in E. coli were discussed. In addition, cadmium, zinc, or copper resistance of the cells expressing metallothionein was examined. Cells transformed with the plasmid pEXPMTII and cultured in a medium containing cadmium exhibited tolerance only to cadmium. It was demonstrated that human metallothionein-2 functioned for cadmium detoxification in E. coli. © Oxford University Press Regular Papers nationallicence Escherichia coli nationallicence expression nationallicence human nationallicence metallothionein and tolerance nationallicence Odawara Fumitomo Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut Kurasaki Masaaki Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut Suzuki-Kurasaki Mika Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut Oikawa Shinji Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut Emoto Tadasu Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut Yamasaki Futoshi Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut Arias Ana Rosa Linde Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut Kojima Yutaka Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut The Journal of Biochemistry Oxford University Press 118/6(1995-12), 1131-1137 0021-924X 118:6<1131 1995 118 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124998 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124998 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Odawara Fumitomo Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Kurasaki Masaaki Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Suzuki-Kurasaki Mika Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Oikawa Shinji Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Emoto Tadasu Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Yamasaki Futoshi Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Arias Ana Rosa Linde Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Kojima Yutaka Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 118/6(1995-12), 1131-1137 0021-924X 118:6<1131 1995 118 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford