caa a22 4500 397493983 CHVBK 20180308164516.0 cr unu---uuuuu 161202e199506 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124848 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124848 Tomida Mikio Department of Chemotherapy, Saitama Cancer Center Research Institute, Ina, Saitama 362 Analysis of Recombinant Soluble Mouse D-Factor/LIF Receptor [Elektronische Daten] [Mikio Tomida] The recombinant soluble mouse D-factor/LIF receptor (sD-factor-R) was expressed in COS-7 cells. Scatchard analysis of the bindings of mouse 125I-D-factor and human 125I-D-factor to the sD-factor-R indicated dissociation constants (Kd) of 12 and 0.267 nM, respectively, which were comparable to those of the binding protein in mouse serum. The apparent molecular masses of the sD-factor-R and human D-factor observed by gel filtration chromatography were 150 and 50 kDa, respectively. The size of the sD-factor-R.human D-factor complex was approximately 200 kDa, indicating that D-factor forms a 1:1 complex with the sD-factor-R. The sD-factor-R inhibited the induction of differentiation of mouse myeloid leukemic M1 cells by mouse D-factor by blocking the binding of this factor to the cells. © Oxford University Press Regular Papers nationallicence antagonist nationallicence D-factor nationallicence LJF nationallicence mouse serum nationallicence soluble receptor nationallicence The Journal of Biochemistry Oxford University Press 117/6(1995-06), 1228-1231 0021-924X 117:6<1228 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124848 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124848 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Tomida Mikio Department of Chemotherapy, Saitama Cancer Center Research Institute, Ina, Saitama 362 NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/6(1995-06), 1228-1231 0021-924X 117:6<1228 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford