caa a22 4500 397494009 CHVBK 20180308164516.0 cr unu---uuuuu 161202e199512 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a125008 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a125008 A 35 kDa Mannose-Binding Lectin with Hemagglutinating and Mitogenic Activities from "Kidachi Aloe” Aloe arborescens Miller var. natalensis Berger [Elektronische Daten] [Takaaki Koike, Hidehiko Beppu, Hiroshi Kuzuya, Kazuhiro Maruta, Kan Shimpo, Masami Suzuki, Koiti Titani, Keisuke Fujita] A novel lectin was isolated from the leaf skin of "Kidachi Aloe” Aloe arborescens Miller var. natalensis Berger by sequential chromatographies on Sephadex G-25 gel filtration, DEAE ion exchange, and Superdex 75 gel filtration columns. The native lectin exhibited a molecular mass of about 35 kDa on both gel filtration on a Superdex 75 column and native-PAGE under nonreducing conditions. SDS-PAGE in the presence or absence of β-mercaptoethanol revealed two distinct peptides with molecular masses of about 5.5 and 2.3 kDa, respectively, in addition to a major 9.2 kDa subunit, indicating the presence of a partially processed subunit. The N-terminal amino acid sequence of the intact subunit showed homology with that of snowdrop lectin. The native lectin showed hemagglutinating activity toward rabbit but not human and sheep erythrocytes, and specifically bound to mannose like snowdrop lectin did, indicating that the Aloe and snowdrop lectins are structurally and functionally similar proteins. In addition, the native lectin showed strong mitogenic activity toward mouse lymphocytes. © Oxford University Press Regular Papers nationallicence Aloe nationallicence amino acid sequence nationallicence hemagglutination nationallicence lectin nationallicence mitogenic activity nationallicence Koike Takaaki Institute of Pharmacognosy, Fujita Health University Hisai, Mie 514-12 aut Beppu Hidehiko Institute of Pharmacognosy, Fujita Health University Hisai, Mie 514-12 aut Kuzuya Hiroshi Divisions of Molecular Biology and istry, Schoolof Health Sciences, Fujita Health University Toyoake, Aichi 470-11 aut Maruta Kazuhiro Department of Biochemistry, School of Health Sciences, Fujita Health University Toyoake, Aichi 470-11 aut Shimpo Kan Institute of Pharmacognosy, Fujita Health University Hisai, Mie 514-12 aut Suzuki Masami Divisons of Biomedical Polymer Science, Institute for Comprehensive Medical Science, Sciences, Fujita Health University University, Toyoake, Aichi 470-11 aut Titani Koiti Divisons of Biomedical Polymer Science, Institute for Comprehensive Medical Science, Sciences, Fujita Health University University, Toyoake, Aichi 470-11 aut Fujita Keisuke Institute of Pharmacognosy, Fujita Health University Hisai, Mie 514-12 aut The Journal of Biochemistry Oxford University Press 118/6(1995-12), 1205-1210 0021-924X 118:6<1205 1995 118 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a125008 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a125008 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koike Takaaki Institute of Pharmacognosy, Fujita Health University Hisai, Mie 514-12 aut NATIONALLICENCE 700 1- Beppu Hidehiko Institute of Pharmacognosy, Fujita Health University Hisai, Mie 514-12 aut NATIONALLICENCE 700 1- Kuzuya Hiroshi Divisions of Molecular Biology and istry, Schoolof Health Sciences, Fujita Health University Toyoake, Aichi 470-11 aut NATIONALLICENCE 700 1- Maruta Kazuhiro Department of Biochemistry, School of Health Sciences, Fujita Health University Toyoake, Aichi 470-11 aut NATIONALLICENCE 700 1- Shimpo Kan Institute of Pharmacognosy, Fujita Health University Hisai, Mie 514-12 aut NATIONALLICENCE 700 1- Suzuki Masami Divisons of Biomedical Polymer Science, Institute for Comprehensive Medical Science, Sciences, Fujita Health University University, Toyoake, Aichi 470-11 aut NATIONALLICENCE 700 1- Titani Koiti Divisons of Biomedical Polymer Science, Institute for Comprehensive Medical Science, Sciences, Fujita Health University University, Toyoake, Aichi 470-11 aut NATIONALLICENCE 700 1- Fujita Keisuke Institute of Pharmacognosy, Fujita Health University Hisai, Mie 514-12 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 118/6(1995-12), 1205-1210 0021-924X 118:6<1205 1995 118 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford