caa a22 4500 397494076 CHVBK 20180308164516.0 cr unu---uuuuu 161202e199508 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124929 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124929 Purification and Phosphorylation of a Mr 25, 000 Protein, an Effective Phosphate Acceptor for Casein Kinase II and Protein Kinase C, Detected in the Cytosolic Fraction of Xenopus laevis Oocytes [Elektronische Daten] [Eikichi Hashimoto, Fumito Takeuchi, Yukie Tanaka, Hirohei Yamamura] A common and effective phosphate acceptor protein for casein kinase II and Ca2+phospholipid-dependent protein kinase (protein kinase C) was purified to near homogeneity from the cytosolic fraction of Xenopus laevis ppcytes. Its molecular mass was estimated to be approximately 25, 000 by SDS-polyacrylamide slab gel electrophoresis and gel filtration analyses. About 1and 2 mol of phosphate were incorporated per mol of this protein with casein kinase II and protein kinase C, respectively, and the phosphorylated amino acid was identified as serine irrespective of the protein kinase employed. The Km values were calculated to be 1 and 0.5 μM for this Mr 25, 000 protein with casein kinase II and protein kinase C, respectively. However, this protein was a relatively poor substrate for casein kinase 1 and did not serve as one for cAMP-dependent protein kinase. The amino acid sequence of its amino-terminal region suggests that this protein is a newly identified substrate for these two protein serine/threonine kinases. © by the Journal of Biochemistry Regular Papers nationallicence casein kinase II nationallicence protein kinase C nationallicence protein phosphorylation nationallicence Xenopus laevis oocyte nationallicence Hashimoto Eikichi Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut Takeuchi Fumito Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut Tanaka Yukie Central Research Laboratories, Fukui Medical School Matsuoka, Fukui 910-11 aut Yamamura Hirohei Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut The Journal of Biochemistry Oxford University Press 118/2(1995-08), 453-460 0021-924X 118:2<453 1995 118 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124929 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124929 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hashimoto Eikichi Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut NATIONALLICENCE 700 1- Takeuchi Fumito Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut NATIONALLICENCE 700 1- Tanaka Yukie Central Research Laboratories, Fukui Medical School Matsuoka, Fukui 910-11 aut NATIONALLICENCE 700 1- Yamamura Hirohei Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 118/2(1995-08), 453-460 0021-924X 118:2<453 1995 118 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford