caa a22 4500 397494084 CHVBK 20180308164516.0 cr unu---uuuuu 161202e199502 xx s 000 0 eng 10.1093/jb/117.2.447 doi (NATIONALLICENCE)oxford-10.1093/jb/117.2.447 Isolation and Characterization of a SCT1 Gene Which Can Suppress a Choline-Transport Mutant of Saccharomyces cerevisiae [Elektronische Daten] [Masaki Matsushita, Jun-ichi Nikawa] The yeast Saccharomyces cerevisiae possesses a choline-transport system encoded by the CTR1 gene. We isolated a yeast gene, SCT1, that can suppress a CTR1 defect when introduced as a multicopy. The SCT1 coding frame is capable of encoding 759 residues with a calculated molecular weight of 85,734. On Northern blot analysis, an RNA species that hybridized with the coding region was detected in the total RNA of the wild-type yeast. The transcription of SCT1 is constitutive. The primary translation product contains three membrane-spanning domains, a PEST-like sequence, and a glutamic acid-rich sequence at the C terminal end. Gene disruption experiments showed that SCT1 is not an essential gene under the standard culture conditions. SCT1 did not suppress a null mutant of ctrl, indicating that a mutant form of choline transporter is necessary for the suppression caused by SCT1. © BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence choline transport nationallicence glutamic acid-rich sequence nationallicence Saccharomyces cerevisiae nationallicence Matsushita Masaki Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, lizuka, Fukuoka 820 aut Nikawa Jun-ichi Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, lizuka, Fukuoka 820 aut The Journal of Biochemistry Oxford University Press 117/2(1995-02), 447-451 0021-924X 117:2<447 1995 117 jbchem https://doi.org/10.1093/jb/117.2.447 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jb/117.2.447 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsushita Masaki Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, lizuka, Fukuoka 820 aut NATIONALLICENCE 700 1- Nikawa Jun-ichi Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, lizuka, Fukuoka 820 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/2(1995-02), 447-451 0021-924X 117:2<447 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford