caa a22 4500 397494165 CHVBK 20180308164516.0 cr unu---uuuuu 161202e199501 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124708 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124708 Clustering of Sp1 Sites Near the Promoter Region of ICP34.5 in Herpes Simplex Virus Type 1 [Elektronische Daten] [In Kwon Chung, Stephen M. Soisson, Mark T. Muller] We report that a host nuclear protein of approximately 100 kDa binds to the tandemly reiterated DR2 sequence of herpes simplex virus type 1 (HSV-1). The DR2 sequence is a repeated component in the "a” sequence, which defines the signals for cleavage and encapsidation of viral DNA; the "a” sequence also contains the promoter regulatory signals for the gene encoding the viral neurovirulence factor, ICP34.5. Characterization of the host binding protein by means of gel shifts and DNase I footprinting revealed this protein is the eukaryotic transcription factor, Sp1. Furthermore, as judged from the sequence homology, the DR2 region contains clustered matches to the consensus binding site for Sp1. Comparison of the host factor and purified Sp1 (by means of gel shifts and footprinting) confirmed these findings. Since clustered DNA recognition elements represent unusually high affinity binding sites, these repeated Sp1 motifs proximal to the ICP34.5 gene suggest that this region may be a major Sp1 binding site in the viral genome. © 1995 BY THE JOURNAL OF BIOCHEMISTRY Rapid Communication nationallicence DNA recognition element nationallicence herpes simplex virus-1 nationallicence Sp1 binding sequence nationallicence transcription factor nationallicence viral neurovirulence gene nationallicence Chung In Kwon Department of Biology, College of Science, Yonsei University Seoul 120-749, Korea aut Soisson Stephen M. Department of Molecular Genetics, The Ohio State University Columbus, OH 43210, USA aut Muller Mark T. Department of Molecular Genetics, The Ohio State University Columbus, OH 43210, USA aut The Journal of Biochemistry Oxford University Press 117/1(1995-01), 19-22 0021-924X 117:1<19 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124708 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124708 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chung In Kwon Department of Biology, College of Science, Yonsei University Seoul 120-749, Korea aut NATIONALLICENCE 700 1- Soisson Stephen M. Department of Molecular Genetics, The Ohio State University Columbus, OH 43210, USA aut NATIONALLICENCE 700 1- Muller Mark T. Department of Molecular Genetics, The Ohio State University Columbus, OH 43210, USA aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/1(1995-01), 19-22 0021-924X 117:1<19 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford