caa a22 4500 397494173 CHVBK 20180308164516.0 cr unu---uuuuu 161202e199506 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124854 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124854 Molecular Cloning and In Situ Localization in the Brain of Rat Sky Receptor Tyrosine Kinase [Elektronische Daten] [Kazumasa Ohashi, Shigeyuki Honda, Noriko Ichinomiya, Toshikazu Nakamura, Kensaku Mizuno] We previously identified a human cDNA encoding a novel receptor tyrosine kinase, termed Sky, which is predominantly expressed in the brain and has a unique extracellular domain consisting of two immunoglobulin (Ig)-like and two fibronectin type HI (FN III) motifs. In attempts to define the functional role of the Sky receptor, we cloned a rat sky cDNA, and localized the sites of expression of sky transcript in the adult rat brain by Northern blot and in situ hybridization analyses using the cloned rat cDNA as a probe. The deduced amino acid sequence of rat Sky has an overall sequence and a domain topology highly conserved with human Sky (90% overall identity and 98% identity within the tyrosine kinase domain). Northern blot analysis revealed that a single 3.8-kb sky mRNA is expressed in PC12 pheochromocytoma and Neuro-2a neuroblastoma cell lines and in various regions of the adult rat brain. In situ hybridization analysis revealed widespread but confined neuronal populations in adult rat brain that express sky transcript; prominent hybridization signals were detected in the inner granular layer of the olfactory bulb, CA-1 area of the hippocampus, granule cell layer of the cerebellum, tenia tectum and cingulate gyms neurons, and wide regions of cortex layers II-VI. The high level of expression of sky mRNA in neurons in restricted brain regions suggests that the Sky receptor may play an important role in development, function, and maintenance of specific neuronal populations in the central nervous system. © Oxford University Press Regular Papers nationallicence in situ hybridization nationallicence neuron nationallicence PC12 cells nationallicence receptor tyrosine kinase nationallicence Sky receptor nationallicence Ohashi Kazumasa Department of Biology, Faculty of Science, Kyushu University Higashi-ku, Fukuoka 812 aut Honda Shigeyuki Biomedical Research Center, Osaka University Medical School Suita, Osaka 565 aut Ichinomiya Noriko Department of Biology, Faculty of Science, Kyushu University Higashi-ku, Fukuoka 812 aut Nakamura Toshikazu Biomedical Research Center, Osaka University Medical School Suita, Osaka 565 aut Mizuno Kensaku Department of Biology, Faculty of Science, Kyushu University Higashi-ku, Fukuoka 812 aut The Journal of Biochemistry Oxford University Press 117/6(1995-06), 1267-1275 0021-924X 117:6<1267 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124854 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124854 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohashi Kazumasa Department of Biology, Faculty of Science, Kyushu University Higashi-ku, Fukuoka 812 aut NATIONALLICENCE 700 1- Honda Shigeyuki Biomedical Research Center, Osaka University Medical School Suita, Osaka 565 aut NATIONALLICENCE 700 1- Ichinomiya Noriko Department of Biology, Faculty of Science, Kyushu University Higashi-ku, Fukuoka 812 aut NATIONALLICENCE 700 1- Nakamura Toshikazu Biomedical Research Center, Osaka University Medical School Suita, Osaka 565 aut NATIONALLICENCE 700 1- Mizuno Kensaku Department of Biology, Faculty of Science, Kyushu University Higashi-ku, Fukuoka 812 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/6(1995-06), 1267-1275 0021-924X 117:6<1267 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford