caa a22 4500 397494181 CHVBK 20180308164516.0 cr unu---uuuuu 161202e199512 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a125004 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a125004 Direct Evidence of Macrophage Differentiation from Bone Marrow Cells in the Liver: A Possible Origin of Kupffer Cells [Elektronische Daten] [Ryu-ichi Takezawa, Yoshifumi Watanabe, Toshihiro Akaike] Controversy has surrounded origin and differentiation of tissue macrophages. We directly demonstrate the differentiation of bone marrow cells into macrophages in the liver in vivo using a cell-labeling fluorescence dye, PKH-26. Bone marrow cells labeled with PKH26 were intravenously injected into syngenic mice, and these cells were tracked by flow cytometric analysis. The majority of the labeled cells were detected only in the liver after 4 days. Interestingly, antigens specific for macrophage lineage cells (F4/80, FCγRII+ and CD14) were detected on the liver-accumulated cells only 4 h after the injection. The pattern of the antigen expression changed to that of Kupffer cells (F4/80+, FcγRII+, Mac-1−) after 4 days and remained so thereafter. These labeled cells in the liver were esterase staining-positive and showed phagocytic activity at day 7. The number of labeled cells among the Kupffer cells ins the liver increased with days after injection. This indicates that bone marrow cells accumulate in the liver and differentiate into liver macrophages on site. Roles of factors secreted from hepatocytes are also discussed. © Oxford University Press Regular Papers nationallicence Kupffer cells nationallicence liver nationallicence macrophage differentiation nationallicence macrophage proliferation nationallicence PKH-26 nationallicence Takezawa Ryu-ichi Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226 aut Watanabe Yoshifumi Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226 aut Akaike Toshihiro Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226 aut The Journal of Biochemistry Oxford University Press 118/6(1995-12), 1175-1183 0021-924X 118:6<1175 1995 118 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a125004 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a125004 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takezawa Ryu-ichi Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Watanabe Yoshifumi Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Akaike Toshihiro Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 118/6(1995-12), 1175-1183 0021-924X 118:6<1175 1995 118 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford