caa a22 4500 39749419X CHVBK 20180308164516.0 cr unu---uuuuu 161202e199503 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124731 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124731 Ordered Structure of the Crystallized Bovine 20S Proteasome [Elektronische Daten] [Yukio Morimoto, Tsunehiro Mizushima, Akiko Yagi, Nobuyuki Tanahashi, Keiji Tanaka, Akira Ichihara, Tomitake Tsukihara] Eukaryotic proteasomes are multicatalytic proteinase complexes with a molecular weight of 750 kDa, containing, respectively, two copies of a hetero-heptamer of α-type subunits and one of β-type subunits, (α1-7β1-7)2. Proteasome was purified from bovine liver and crystallized into a hexagonal system with cell dimensions of a= 6 = 121.83(2) Å, c = 930.68(6) Å. A cylindrical particle size of 122 Å diameter and 155 Å height was determined from the molecular packing in a unit cell. The crystal gave diffraction spots up to at least 4.4 Å resolution, which was the minimum spacing of the camera used. The overall temperature factor of the enzyme was estimated to be in the range of 36.2 to 25.8 Å2. These results imply that the enzyme complex has a unique ordered structure comprising multisubunits with two types of hetero-heptamer. This ordered structure may facilitate highly organized cooperation of individual functions of subunits within the enzyme complex. © 1995 BY THE JOURNAL OF BIOCHEMISTRY Rapid Communication nationallicence crystal nationallicence eukaryotic proteasome nationallicence X-ray diffraction nationallicence Morimoto Yukio Faculty of Engineering, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut Mizushima Tsunehiro Faculty of Engineering, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut Yagi Akiko Faculty of Engineering, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut Tanahashi Nobuyuki Institute for Enzyme Research, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut Tanaka Keiji Institute for Enzyme Research, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut Ichihara Akira Institute for Enzyme Research, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut Tsukihara Tomitake Faculty of Engineering, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut The Journal of Biochemistry Oxford University Press 117/3(1995-03), 471-474 0021-924X 117:3<471 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124731 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124731 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morimoto Yukio Faculty of Engineering, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut NATIONALLICENCE 700 1- Mizushima Tsunehiro Faculty of Engineering, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut NATIONALLICENCE 700 1- Yagi Akiko Faculty of Engineering, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut NATIONALLICENCE 700 1- Tanahashi Nobuyuki Institute for Enzyme Research, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut NATIONALLICENCE 700 1- Tanaka Keiji Institute for Enzyme Research, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut NATIONALLICENCE 700 1- Ichihara Akira Institute for Enzyme Research, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut NATIONALLICENCE 700 1- Tsukihara Tomitake Faculty of Engineering, The University of Tokushima 2-1 Minami-Josanjima, Tokushima, Tokushima 770 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/3(1995-03), 471-474 0021-924X 117:3<471 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford