caa a22 4500 397494211 CHVBK 20180308164516.0 cr unu---uuuuu 161202e199504 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124786 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124786 Structural Composition of Hammerhead Ribozymes [Elektronische Daten] [Akio Takenaka, Osamu Matsumoto, Yixin Chen, Sei-ichi Hasegawa, Toshiyuki Chatake, Masaru Tsunoda, Tsutomu Ohta, Yasuo Komatsu, Makoto Koizumi, Eiko Ohtsuka] Eleven kinds of hammerhead ribozymes were designed and synthesized to investigate the structural composition of the complexes and to find suitable crystallization conditions, the substrate chains having been modified to prevent hydrolysis. Electrophoresis patterns indicated that the two strands except for the substrate chain form a binary complex and that they form a ternary complex when mixed with the substrate chain. Both complexes were crystallized. The crystal of the binary complex belongs to a Laue symmetry of 32 (space group of P321, P3121, or P3221) with cell dimensions of a=b=53.4 and c=59.4 Å. The volume per one nucleotide allows the asymmetric unit to contain one binary complex. Our results suggest that the catalytic part forms a rigid ribozyme structure which induces a scissile reaction when the substrate is bound, in a similar manner to an enzyme protein. © Oxford University Press Regular Papers nationallicence crystallization nationallicence electrophoresis nationallicence ribozyme nationallicence X-ray work nationallicence Takenaka Akio Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Nagatsuda 4259, Midori-ku, Yokohama 226 aut Matsumoto Osamu Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Nagatsuda 4259, Midori-ku, Yokohama 226 aut Chen Yixin Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Nagatsuda 4259, Midori-ku, Yokohama 226 aut Hasegawa Sei-ichi Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Nagatsuda 4259, Midori-ku, Yokohama 226 aut Chatake Toshiyuki Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Nagatsuda 4259, Midori-ku, Yokohama 226 aut Tsunoda Masaru Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Nagatsuda 4259, Midori-ku, Yokohama 226 aut Ohta Tsutomu Faculty of Pharmaceutical Sciences, Hokkaido University Kita-12, Nishi-6, Kita-ku, Sapporo 060 aut Komatsu Yasuo Faculty of Pharmaceutical Sciences, Hokkaido University Kita-12, Nishi-6, Kita-ku, Sapporo 060 aut Koizumi Makoto Faculty of Pharmaceutical Sciences, Hokkaido University Kita-12, Nishi-6, Kita-ku, Sapporo 060 aut Ohtsuka Eiko Faculty of Pharmaceutical Sciences, Hokkaido University Kita-12, Nishi-6, Kita-ku, Sapporo 060 aut The Journal of Biochemistry Oxford University Press 117/4(1995-04), 850-855 0021-924X 117:4<850 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124786 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124786 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takenaka Akio Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Nagatsuda 4259, Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Matsumoto Osamu Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Nagatsuda 4259, Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Chen Yixin Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Nagatsuda 4259, Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Hasegawa Sei-ichi Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Nagatsuda 4259, Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Chatake Toshiyuki Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Nagatsuda 4259, Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Tsunoda Masaru Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology Nagatsuda 4259, Midori-ku, Yokohama 226 aut NATIONALLICENCE 700 1- Ohta Tsutomu Faculty of Pharmaceutical Sciences, Hokkaido University Kita-12, Nishi-6, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Komatsu Yasuo Faculty of Pharmaceutical Sciences, Hokkaido University Kita-12, Nishi-6, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Koizumi Makoto Faculty of Pharmaceutical Sciences, Hokkaido University Kita-12, Nishi-6, Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Ohtsuka Eiko Faculty of Pharmaceutical Sciences, Hokkaido University Kita-12, Nishi-6, Kita-ku, Sapporo 060 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/4(1995-04), 850-855 0021-924X 117:4<850 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford