caa a22 4500 39749422X CHVBK 20180308164516.0 cr unu---uuuuu 161202e199510 xx s 000 0 eng 10.1093/jb/118.5.1045 doi (NATIONALLICENCE)oxford-10.1093/jb/118.5.1045 Function of RNH-l/14-3-3β Gene in Cellular Differentiation and Proliferation [Elektronische Daten] [Rina Takai, Eriko Tanaka, Toshiaki Miyazaki, Mikiya Suda, Fumio Tashiro] It has recently been reported that the members of 14-3-3 protein family participate in cell cycle control and associate with Raf, Bcr, Bcr-Abl, and polyomavirus middle tumor antigen (MT) as modulators of signal transduction. During cDNA cloning for the 17-kDa neuronal differentiation factor (K2 factor) secreted from rat hepatoma Kagura-2 (K2) cells from a K2 cDNA library using rat prepronerve growth factor (prepro NGF) cDNA as a probe, we obtained RNH-1 (rat NGF homologue) clone, which was identified as 14-3-3β cDNA, but not K2 factor, although no significant homology is present between 14-3-3β and prepro NGF cDNAs. RNH-1/14-3-3β gene was markedly expressed as a 2.9-kb mRNA in K2 cells and in newborn rat brain tissue. In PC12 cells the expression of this gene was down-regulated during the neuronal differentiation primed by NGF. The enforced expression of RNH-1/14-3-3β in PC12 and K2 cells conferred on them a higher sensitivity to NGF for neuronal differentiation and an intense growth ability in low serum medium, respectively. These results provide additional evidence that RNH-l/14-3-3β protein participates in cellular differentiation, proliferation and transformation through the signal transduction pathways of various growth factors. © Oxford University Press Regular Papers nationallicence Takai Rina Department of Biological Science and Technology, Science University of Tokyo, Noda, Chiba 278 aut Tanaka Eriko Department of Biological Science and Technology, Science University of Tokyo, Noda, Chiba 278 aut Miyazaki Toshiaki Department of Biological Science and Technology, Science University of Tokyo, Noda, Chiba 278 aut Suda Mikiya Department of Biological Science and Technology, Science University of Tokyo, Noda, Chiba 278 aut Tashiro Fumio Department of Biological Science and Technology, Science University of Tokyo, Noda, Chiba 278 aut The Journal of Biochemistry Oxford University Press 118/5(1995-10), 1045-1053 0021-924X 118:5<1045 1995 118 jbchem https://doi.org/10.1093/jb/118.5.1045 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jb/118.5.1045 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takai Rina Department of Biological Science and Technology, Science University of Tokyo, Noda, Chiba 278 aut NATIONALLICENCE 700 1- Tanaka Eriko Department of Biological Science and Technology, Science University of Tokyo, Noda, Chiba 278 aut NATIONALLICENCE 700 1- Miyazaki Toshiaki Department of Biological Science and Technology, Science University of Tokyo, Noda, Chiba 278 aut NATIONALLICENCE 700 1- Suda Mikiya Department of Biological Science and Technology, Science University of Tokyo, Noda, Chiba 278 aut NATIONALLICENCE 700 1- Tashiro Fumio Department of Biological Science and Technology, Science University of Tokyo, Noda, Chiba 278 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 118/5(1995-10), 1045-1053 0021-924X 118:5<1045 1995 118 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford